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Current Microbiology

, Volume 30, Issue 2, pp 97–103 | Cite as

Bacteria produce the volatile hydrocarbon isoprene

  • Jennifer Kuzma
  • Michele Nemecek-Marshall
  • Walter H. Pollock
  • Ray Fall
Article

Abstract

Various bacterial species, both Gram-negative and Gram-positive, were found to produce the volatile hydrocarbon isoprene (2-methyl-1,3-butadiene). Out of the tested cultures, Bacillus produced the most isoprene. The production of isoprene from bacteria was confirmed by gas chromatography-mass spectrometry. Media and growth effects on isoprene production were investigated: growth in rich media led to higher levels of isoprene than growth in minimal media, and highest isoprene emission rates were seen in log-phase cultures. Temperature profiles for bacterial isoprene production showed an optimum of 45°C and were suggestive of an enzymatic mechanism for isoprene formation.

Keywords

Hydrocarbon Bacillus Temperature Profile Bacterial Species Minimal Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1945

Authors and Affiliations

  • Jennifer Kuzma
    • 1
  • Michele Nemecek-Marshall
    • 1
  • Walter H. Pollock
    • 2
  • Ray Fall
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of ColoradoBoulderUSA
  2. 2.National Center for Atmospheric ResearchBoulderUSA

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