, Volume 91, Issue 2, pp 108–112 | Cite as

Silver staining of meiotic chromosomes in the fungus, Coprinus cinereus

  • Patricia J. Pukkila
  • Benjamin C. Lu


We have taken advantage of the synchronous meiotic process in the basidiomycete Coprinus cinereus to develop a simple and rapid method to selectively stain meiotic chromosomes and nucleoli in this fungus without prior removal of the cell wall. Electron microscopic examination of these silver-stained chromosomes indicated that the lateral elements of the synaptonemal complexes were prominently stained, and terminal attachment plaques were apparent. We found that a translocation quadrivalent could be recognized easily in the light microscope using these methods. The procedures appear suitable for the characterization of chromosome rearrangements in this small genome, and should facilitate cytogenetic analysis in this fungus.


Cell Wall Light Microscope Developmental Biology Microscopic Examination Rapid Method 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Patricia J. Pukkila
    • 1
  • Benjamin C. Lu
    • 2
  1. 1.Department of Biology and Curriculum in GeneticsUniversity of North CarolinaChapel HillUSA
  2. 2.Department of Molecular Biology and GeneticsUniversity of GuelphGuelphCanada

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