Molecular and General Genetics MGG

, Volume 182, Issue 3, pp 440–444 | Cite as

Isolation and characterization of a genomic sequence of maize coding for a zein gene

  • Udo Wienand
  • Peter Langridge
  • Günter Feix
Article

Summary

An EcoRI restriction endonuclease fragment of maize DNA coding for the 19,000 dalton zein protein was cloned in phage λgt WES. The zein gene was identified by the electron microscopic analysis of RNA-DNA hybrids (R-loops) and DNA-DNA hybrids (D-loops). The R-loops were formed with poly(rA)-containing RNA isolated from 18 days post-pollination maize endosperm and showed no intervening non-hybridizing sequences (introns) within their 800 base length. A cDNA clone specific for the 19,000 dalton zein protein formed D-loops in the same position and orientation as the R-loops. The cloned fragment measured 4.4 kilobases (kb), the same size as an EcoRI fragment of maize DNA revealed by “Southern” analysis.

Keywords

Maize Genomic Sequence Restriction Endonuclease cDNA Clone Microscopic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Udo Wienand
    • 1
  • Peter Langridge
    • 1
  • Günter Feix
    • 1
  1. 1.Institut für Biologie IIIUniversität FreiburgFreiburgFederal Republic of Germany

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