Molecular and General Genetics MGG

, Volume 247, Issue 4, pp 391–398 | Cite as

Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis

  • Toshisuke Iwasaki
  • Kazuko Yamaguchi-Shinozaki
  • Kazuo Shinozaki
Original Paper

Abstract

In Arabidopsis thaliana, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA) but the gene does not include any sequence corresponding to the consensus ABA-responsive element (ABRE), RYACGTGGYR, in its promoter region. The cis-regulatory region of the rd22 promoter was identified by monitoring the expression of β-glucuronidas (GUS) activity in leaves of transgenic tobacco plants transformed with chimeric gene fusions constructed between 5′-deleted promoters of rd22 and the coding region of the GUS reporter gene. A 67-bp nucleotide fragment corresponding to positions − 207 to − 141 of the rd22 promoter conferred responsiveness to dehydration and ABA on a non-responsive promoter. The 67-bp fragment contains the sequences of the recognition sites for some transcription factors, such as MYC, MYB, and GT-1. The fact that accumulation of rd22 mRNA requires protein synthesis raises the possibility that the expression of rd22 might be regulated by one of these trans-acting protein factors whose de novo synthesis is induced by dehydration or ABA. Although the structure of the RD22 protein is very similar to that of a non-storage seed protein, USP, of Vicia faba, the expression of the GUS gene driven by the rd22 promoter in non-stressed transgenic Arabidopsis plants was found mainly in flowers and bolted stems rather than in seeds.

Key words

Arabidopsis thaliana Dehydration-responsive gene Abscisic acid Promoter β-Glucuronidase 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Toshisuke Iwasaki
    • 1
  • Kazuko Yamaguchi-Shinozaki
    • 1
    • 2
  • Kazuo Shinozaki
    • 1
  1. 1.Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN)Tsukuba Life Science CenterTsukuba, IbarakiJapan
  2. 2.Biological Resources DivisionJapan International Research Center for Agricultural Sciences (JIRCAS)Tsukuba, IbarakiJapan

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