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Molecular and General Genetics MGG

, Volume 125, Issue 1, pp 9–52 | Cite as

Mitochondrial genetics

IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae
  • P. R. Avner
  • D. Coen
  • B. Dujon
  • P. P. Slonimski
Article

Summary

  1. 1.

    Two loci OIand OIIlocalized on the mitochondrial DNA and conferring oligomycin resistance in S. cerevisiae have been demonstrated. The two loci show a high frequency of recombination with each other.

     
  2. 2.

    In the majority of OR strains examined the mutation was found to map at the OIlocus.

     
  3. 3.

    Mapping studies have indicated that the OIand OIIloci are either unlinked or very weakly linked to each other. Both of these loci also appear to be essentially unlinked from the ω-RI-RII-RIIIsegment of the mitochondrial genome specifying mitoribosomal functions.

     
  4. 4.

    Analysis of crosses involving mutants at either the OIor OIIloci and a series of ϱ petites variously deleted in known mitochondrial genes has demonstrated that the two oligomycin resistance loci OIand OIIare separable. Assuming that the non deleted segment of the mitochondrial DNA is continuous, the results suggest the gene order ω-RI-RII-RIII-OI-OII.

     
  5. 5.

    The rules applicable to the system and previously delineated from studies of the RI, RIIand RIIIloci in oligomycin sensitive strains have been shown to apply equally to strains carrying OR alleles at the OIand OIIloci. Oligomycin resistance alleles at both the O loci, OIand OII, are present in both ω+ and ω strains.

     
  6. 6.

    The presence of a nuclear gene(s) in a strain D6, influencing certain facets of the recombination process in both homosexual and heterosexual crosses has been demonstrated and this behaviour is superimposed upon, and clearly separable from the effects due to the mitochondrial gene ω.

     

Keywords

Recombination Mitochondrial Genome Nuclear Gene Mitochondrial Gene Gene Order 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CS/CR

Allelic forms of the RIlocus conferring chloramphenicol sensitivity/resistance

ES/ER

Allelic forms of either the RIIor RIIIloci conferring erythromycin sensitivity/resistance

OS/OR

Allelic forms of either of the loci conferring oligomycin sensitivity/resistance

OIand OII

Non allelic mitochondrial loci conferring oligomycin resistance

RI

The mitochondrial locus conferring chloramphenicol resistance

RIIand RIII

Non allelic mitochondrial loci conferring erythromycin resistance

ω+

Allelic forms of the mitochondrial sex factor

ϱ+

“grande” or respiratory competent cells

ϱ

“petite” or cytoplasmically inherited respiratory deficient cells

C0, E0, O0

Deletions for the antibiotic resistance genes C, E and O

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • P. R. Avner
    • 1
  • D. Coen
    • 2
  • B. Dujon
    • 2
  • P. P. Slonimski
    • 2
  1. 1.Department of Molecular SciencesUniversity of WarwickCoventryEngland
  2. 2.Centre de Génétique Moléculaire du Centre National de la Recherche ScientifiqueGif sur YvetteFrance

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