Molecular and General Genetics MGG

, Volume 231, Issue 3, pp 489–493 | Cite as

Homology of the root adhesin of Pseudomonas fluorescens OE 28.3 with porin F of P. neruginosa and P. syringae

  • R. De Mot
  • P. Proost
  • J. Van Damme
  • J. Vanderleyden
Short Communications


The gene encoding the root adhesin from the outer membrane of Pseudomonas fluorescens OE 28.3 was isolated from a genomic λEMBL3 library and sequenced. The deduced protein (32104 daltons) displayed strong homology with the amino- and carboxyterminal parts of porin F (OprF) from P. aeruginosa and P. syringae. Significant homology was also found within the C-terminal domain of the OmpA proteins from Enterobacteria and major outer membrane proteins from Neisseria species. However, a cysteine-rich domain present in the OprFs of P. aeruginosa and P. syringae is absent from the adhesin of P. fluorescens. Instead, it contains a shorter sequence with eight alternating proline residues.

Key words

Plant growth-promoting rhizobacteria Pseudomonas fluorescens Outer membrane protein Adhesin Root colonization 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. De Mot
    • 1
  • P. Proost
    • 2
  • J. Van Damme
    • 2
  • J. Vanderleyden
    • 1
  1. 1.F.A. Janssens Laboratory of GeneticsCatholic University of LeuvenHeverleeelgium
  2. 2.Rega Institute for Medical ResearchCatholic University of LeuvenLeuvenBelgium

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