Advertisement

Molecular and General Genetics MGG

, Volume 231, Issue 3, pp 489–493 | Cite as

Homology of the root adhesin of Pseudomonas fluorescens OE 28.3 with porin F of P. neruginosa and P. syringae

  • R. De Mot
  • P. Proost
  • J. Van Damme
  • J. Vanderleyden
Short Communications

Summary

The gene encoding the root adhesin from the outer membrane of Pseudomonas fluorescens OE 28.3 was isolated from a genomic λEMBL3 library and sequenced. The deduced protein (32104 daltons) displayed strong homology with the amino- and carboxyterminal parts of porin F (OprF) from P. aeruginosa and P. syringae. Significant homology was also found within the C-terminal domain of the OmpA proteins from Enterobacteria and major outer membrane proteins from Neisseria species. However, a cysteine-rich domain present in the OprFs of P. aeruginosa and P. syringae is absent from the adhesin of P. fluorescens. Instead, it contains a shorter sequence with eight alternating proline residues.

Key words

Plant growth-promoting rhizobacteria Pseudomonas fluorescens Outer membrane protein Adhesin Root colonization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bauw G, Van Damme J, Puype M, Vandekerckhove J, Gesser B, Ratz GP, Lauridsen JB, Celis JE (1989) Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels. Proc Natl Acad Sci USA 86:7701–7705Google Scholar
  2. Beck E, Bremer E (1980) Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12. Nucleic Acids Res 8:3011–3027Google Scholar
  3. Braun G, Cole ST (1982) The nucleotide sequence coding for major outer membrane protein OmpA of Shigella dysenteriae. Nucleic Acids Res 10:2367–2378Google Scholar
  4. Braun G, Cole ST (1983) Molecular characterization of the gene coding for major outer membrane protein OmpA from Enterobacter aerogenes. Eur J Biochem 137:495–500Google Scholar
  5. Braun G, Cole ST (1984) DNA sequence of the Serratia marcescens ompA gene: implications for the organisation of an enterobacterial outer membrane protein. Mol Gen Genet 195:321–328Google Scholar
  6. Charles IG, Dougan G, Pickard D, Chatfield S, Smith M, Novotny P, Morrissey P, Fairweather NF (1989) Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis. Proc Natl Acad Sci USA 86:3554–3558Google Scholar
  7. Dallo SF, Chavoya A, Baseman JB (1990) Characterization of the gene for a 30-kilodalton adhesin-related protein of Mycoplasma pneumoniae. Infect Immun 58:4163–4165Google Scholar
  8. De Mot R, Vanderleyden J (1989) Application of two-dimensional protein analysis for strain fingerprinting and mutant analysis of Azospirillum species. Can J Microbiol 35:960–967Google Scholar
  9. De Mot R, Vanderleyden J (1991) Purification of a root-adhesive outer membrane protein of root-colonizing Pseudomonas fluorescens. FEMS Microbiol Lett 81:323–328Google Scholar
  10. Demuth DR, Golub EE, Malamud D (1990) Streptococcal-host interactions. Structural and functional analysis of a Streptococcus sanguis receptor for a human salivary glycoprotein. J Biol Chem 265:7120–7126Google Scholar
  11. Duchêne M, Schweizer A, Lottspeich F, Krauss G, Marget M, Vogel K, von Specht BU, Domdey H (1988) Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene. J Bacteriol 170:155–162Google Scholar
  12. Evans JS, Levine BA, Trayer IP, Dorman CJ, Higgins CF (1986) Sequence-imposed structural constraints in the TonB protein of E. coli. FEBS Lett 208:211–216Google Scholar
  13. Freudl R, Cole ST (1983) Cloning and molecular characterization of the ompA gene from Salmonella typhimurium. Eur J Biochem 134:497–502Google Scholar
  14. Frischauf AM, Murray N, Lehrach H (1987) Lambda phage vectors — EMBL series. Meth Enzymol 153:103–115Google Scholar
  15. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RAJ (1991) Domains in microbial β-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev 55:303–315Google Scholar
  16. Gotschlich EC, Seiff M, Balke MS (1987) The DNA sequence of the structural gene of gonococcal protein III and the flanking region containing a repetitive sequence. Homology of protein III with the enterobacterial OmpA proteins. J Exp Med 165:471–482Google Scholar
  17. Jones R, Haselkorn R (1989) The DNA sequence of the Rhodobacter capsulatus ntrA, ntrB and ntrC gene analogues required for nitrogen fixation. Mol Gen Genet 215:507–516Google Scholar
  18. Kloepper JW, Lifshitz R, Zablotowicz RM (1989) Free-living bacterial inocula for enhancing crop productivity. Trends in Biotechnol 7:39–44Google Scholar
  19. Klose M, Schwartz H, MacIntyre S, Freudl R, Eschbach M-L, Henning U (1988) Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptide. J Biol Chem 263:13291–13296Google Scholar
  20. Klugman KP, Gotschlich EC, Blake MS (1989) Sequence of the structural gene (rpmM) for the class 4 outer membrane protein of Neisseria meningitidis, homology of the protein to gonococcal protein III and Escherichia coli OmpA, and construction of meningococcal strains that lack class 4 protein. Infect Immun 57:2066–2071Google Scholar
  21. Lerner CG, Inouye M (1990) Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert, screening capability. Nucleic Acids Res 18:4631Google Scholar
  22. Li LJ, Dougan G, Novotny P, Charles IG (1991) P.70 pertactin, an outer-membrane protein from Bordetella parapertussis: cloning, nucleotide sequence and surface expression in Escherichia coli. Mol Microbiol 5:409–417Google Scholar
  23. Lockett TJ (1990) A bacteriophage lambda DNA purification procedure suitable for the analysis of DNA from either large or multiple small lysates. Anal Biochem 185:230–234Google Scholar
  24. MacArthur MW, Thornton JM (1991) Influence of proline residues on protein conformation. J Mol Biol 218:397–412Google Scholar
  25. Meinkoth J, Wahl G (1984) Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138:267–284Google Scholar
  26. Morona R, Klose M, Henning U (1984) Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins. J Bacteriol 159:570–578Google Scholar
  27. Nikaido H, Nikaido K, Harayama S (1991) Identification and characterization of porins in Pseudomonas aeruginosa. J Biol Chem 266:770–779Google Scholar
  28. Schwarz E, Oesterhelt D, Reinke H, Beyreuther K, Dimroth P (1988) The sodium ion translocating oxalacetate decarboxylase of Klebsiella pneumoniae. Sequence of the biotin-containing alpha-subunit and relationship to other biotin-containing enzymes. J Biol Chem 263:9640–9645Google Scholar
  29. Siehnel RJ, Martin NL, Hancock REW (1990) Function and structure of the porin proteins OprF and OprP of Pseudomonas aeruginosa. In: Silver S, Chakrabarty AM, Iglewski B, Kaplan S (eds) Pseudomonas, biotransformations, pathogenesis, and evolving biotechnology, American Society for Microbiology, Washington, DC, pp 328–342Google Scholar
  30. Ullstrom CA, Siehnel R, Woodruff W, Steinbach S, Hancock REW (1991) Conservation of the gene for outer membrane protein OprF in the family Pseudomonadaceae: sequence of the Pseudomonas syringae oprF. J Bacteriol 173:768–775Google Scholar
  31. Vogel H, Jähnig F (1986) Models for the structure of outer-membrane proteins of Escherichia coli derived from Raman spectroscopy and prediction methods. J Mol Biol 190:191–199Google Scholar
  32. White DA, Barlow AK, Clarke IN, Heckels JE (1990) Stable expression of meningococcal class 1 protein in an antigenically reactive form in outer membranes of Escherichia coli. Mol Microbiol 4:769–776Google Scholar
  33. Woodruff WA, Hancock REW (1989) Pseudomonas aeruginosa outer membrane protein F: structural role and relationship to the Escherichia coli OmpA protein. J Bacteriol 171:3304–3309Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. De Mot
    • 1
  • P. Proost
    • 2
  • J. Van Damme
    • 2
  • J. Vanderleyden
    • 1
  1. 1.F.A. Janssens Laboratory of GeneticsCatholic University of LeuvenHeverleeelgium
  2. 2.Rega Institute for Medical ResearchCatholic University of LeuvenLeuvenBelgium

Personalised recommendations