Human Genetics

, Volume 73, Issue 1, pp 27–30

The frequency of false-positive and false-negative results in the detection of Y-chromosomes in interphase nuclei

  • J. L. Thomsen
  • E. Niebuhr
Original Investigations

Summary

In blood smears from 527 females and 457 males examined for the presence of Y chromosomes in interphase nuclei, 0.6% false-positive results and 11% false-negative results were found. There was a clear tendency for the falsenegative results to occur among those with small fluorescent or non-existing bands on the Y chromosome. The three falsepositive females all had fluorescent chromosomal variants. In a comparison between female samples with and without chromosomal variants respectively, the former showed significantly higher false Y-body counts. There was a decrease in the number of Y-bodies with increasing age. There were no significant differences between staining with 0.1% Quinacrine mustard and 0.1% and 1% Mepacrine. This study provides a] more solid basis for the use of Y chromosome detection in forensic medicine, for screening purposes etc.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. L. Thomsen
    • 1
  • E. Niebuhr
    • 1
  1. 1.University Institute of Forensic Medicine and Medical GeneticsCopenhagenDenmark

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