Archives of Toxicology

, Volume 58, Issue 1, pp 5–9 | Cite as

Thiourea induces DNA repair synthesis in primary rat hepatocyte cultures and gene mutations in V79 Chinese hamster cells

  • Kyriakoula Ziegler-Skylakakis
  • Stephan Rossberger
  • Ulrich Andrae
Original Investigations

Abstract

Thiourea was investigated for its capacity to cause DNA alterations in cultured mammalian cells. The induction of DNA repair in primary rat hepatocyte cultures and of gene mutations in V79 Chinese hamster cells were used as biological endpoints. In hepatocytes, thiourea elicited a linear increase in DNA repair replication in the concentration range tested (5–25 mM). In V79 cells, thiourea (10–40 mM) significantly increased the frequency of 8-azaguanine-resistant mutants. The present results show that thiourea is weakly, but definitely, genotoxic and mutagenic in cultured mammalian cells.

Key words

Thiourea Carcinogenicity Mutagenicity Genotoxicity Mammalian cell cultures 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Kyriakoula Ziegler-Skylakakis
    • 1
  • Stephan Rossberger
    • 1
  • Ulrich Andrae
    • 1
  1. 1.Abteilung für ToxikologieGesellschaft für Strahlen- und Umweltforschung (GSF)Neuherberg/MünchenFederal Republic of Germany

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