Advertisement

Diabetologia

, Volume 30, Issue 6, pp 394–396 | Cite as

Haemolysis affects insulin but not C-peptide immunoassay

  • S. O'Rahilly
  • M. A. Burnett
  • R. F. Smith
  • J. H. Darley
  • R. C. Turner
Originals

Summary

Venous blood was taken at the end of a glucose infusion test in 19 individuals and divided into four aliquots, 3 of which were variably haemolysed by repeated passage through a 23-gauge needle to simulate traumatic venepuncture. Plasma insulin (measured by both a charcoal separation and a double-antibody method), C-peptide, and haemoglobin were measured in each aliquot, and haemolysis was also assessed visibly. A significant loss of immuno-assayable plasma insulin was found in samples with only a trace of visible haemolysis, with up to 90% lost in severely haemolysed samples. Plasma C-peptide was unaffected by haemolysis. This represents an additional advantage for the use of plasma C-peptide in assessing insulin secretion.

Key words

Insulin C-peptide radio-immunoassay haemolysis 

References

  1. 1.
    Albano JDM, Ekins RP, Maritz G, Turner RC (1972) A sensitive precise radioimmunoassay of serum insulin relying on charcoal separation bound and free hormone moieties. Acta Endocrinol 70: 487–509Google Scholar
  2. 2.
    Morgan CR, Lazarow A (1961) Immunoassay of insulin: two-antibody system. Plasma insulin levels in normal sub-diabetic and diabetic rats. Diabetes 12: 115–126Google Scholar
  3. 3.
    Holman RR, Turner RC (1977) Diabetes: the quest for basal normoglycaemia. Lancet 1: 469–474Google Scholar
  4. 4.
    Crosby WH, Furth FW (1956) Modification of the benzidine method for measurement of haemoglobin in plasma and urine. Blood 11: 380Google Scholar
  5. 5.
    Bing FC, Baker RW (1931) Determination of haemoglobin in minute amounts of blood by Wu's method. J Biol Chem 92: 589Google Scholar
  6. 6.
    Tager H, Given B, Baldwin D, Mako M, Markese J (1979) A structurally abnormal insulin causing human diabetes. Nature 281: 122–125Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • S. O'Rahilly
    • 1
  • M. A. Burnett
    • 1
  • R. F. Smith
    • 2
  • J. H. Darley
    • 3
  • R. C. Turner
    • 1
  1. 1.Diabetes Research LaboratoriesJohn Radcliffe HospitalOxfordUK
  2. 2.Sheikh Rashid Diabetes UnitJohn Radcliffe HospitalOxfordUK
  3. 3.Department of HaematologyJohn Radcliffe HospitalOxfordUK

Personalised recommendations