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Chromosoma

, Volume 90, Issue 2, pp 128–132 | Cite as

Restriction enzyme banding of mouse metaphase chromosomes

  • Margot Kaelbling
  • Dorothy A. Miller
  • Orlando J. Miller
Article

Abstract

Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Aha I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters.

Keywords

Restriction Endonuclease Developmental Biology Mouse Strain Metaphase Chromosome Small Segment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Margot Kaelbling
    • 1
    • 2
  • Dorothy A. Miller
    • 1
    • 2
  • Orlando J. Miller
    • 1
    • 2
    • 3
  1. 1.Department of Human Genetics and DevelopmentColumbia UniversityNew YorkUSA
  2. 2.Cancer CenterColumbia UniversityNew YorkUSA
  3. 3.Department of Obstetrics and GynecologyColumbia UniversityNew YorkUSA

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