, Volume 90, Issue 2, pp 128–132 | Cite as

Restriction enzyme banding of mouse metaphase chromosomes

  • Margot Kaelbling
  • Dorothy A. Miller
  • Orlando J. Miller


Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Aha I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters.


Restriction Endonuclease Developmental Biology Mouse Strain Metaphase Chromosome Small Segment 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Margot Kaelbling
    • 1
    • 2
  • Dorothy A. Miller
    • 1
    • 2
  • Orlando J. Miller
    • 1
    • 2
    • 3
  1. 1.Department of Human Genetics and DevelopmentColumbia UniversityNew YorkUSA
  2. 2.Cancer CenterColumbia UniversityNew YorkUSA
  3. 3.Department of Obstetrics and GynecologyColumbia UniversityNew YorkUSA

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