Mammalian Genome

, Volume 3, Issue 5, pp 274–280 | Cite as

The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes

  • Nobuyuki Mizuki
  • Donna E. Sarapata
  • Jose A. Garcia-Sanz
  • Masanori Kasahara
Original Contributions


Tpx-1 is a testis-specific gene that maps on mouse Chromosome (Chr) 17. The deduced TPX-1 protein shows 55% amino acid sequence similarity to acidic epididymal glycoprotein (AEG), assumed to be involved in sperm maturation. In the present study, we determined the genomic structure of the mouse Tpx-1 gene and the cellular localization of its transcripts. The gene was found to contain ten exons, with an unusually large intron (∼17.0 kilobase pairs) between exons 8 and 9. In situ hybridization of testicular sections showed that Tpx-1 is transcribed abundantly by haploid male germ cells. A computer search of protein databases revealed that deduced TPX-1/AEG proteins have significant sequence similarity (∼30%) to two non-mammalian proteins: “pathogenesis-related” proteins 1 of tobaccos, and venom sac proteins of whiteface hornets, known as Dol m V. Amino acid residues encoded by exon 10 of the Tpx-1 gene and most of those encoded by exon 9 were absent in the non-mammalian proteins. This result suggests that the ancestor of Tpx-1 acquired exons 9 and 10 after its divergence from the ancestors of the plant and insect proteins.


Sequence Similarity Mouse Chromosome Amino Acid Sequence Similarity Male Germ Cell Computer Search 
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Copyright information

© Springer-Verlag New York Inc 1992

Authors and Affiliations

  • Nobuyuki Mizuki
    • 1
  • Donna E. Sarapata
    • 1
  • Jose A. Garcia-Sanz
    • 1
  • Masanori Kasahara
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Miami School of MedicineMiamiUSA

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