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Mammalian Genome

, Volume 3, Issue 5, pp 274–280 | Cite as

The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes

  • Nobuyuki Mizuki
  • Donna E. Sarapata
  • Jose A. Garcia-Sanz
  • Masanori Kasahara
Original Contributions

Abstract

Tpx-1 is a testis-specific gene that maps on mouse Chromosome (Chr) 17. The deduced TPX-1 protein shows 55% amino acid sequence similarity to acidic epididymal glycoprotein (AEG), assumed to be involved in sperm maturation. In the present study, we determined the genomic structure of the mouse Tpx-1 gene and the cellular localization of its transcripts. The gene was found to contain ten exons, with an unusually large intron (∼17.0 kilobase pairs) between exons 8 and 9. In situ hybridization of testicular sections showed that Tpx-1 is transcribed abundantly by haploid male germ cells. A computer search of protein databases revealed that deduced TPX-1/AEG proteins have significant sequence similarity (∼30%) to two non-mammalian proteins: “pathogenesis-related” proteins 1 of tobaccos, and venom sac proteins of whiteface hornets, known as Dol m V. Amino acid residues encoded by exon 10 of the Tpx-1 gene and most of those encoded by exon 9 were absent in the non-mammalian proteins. This result suggests that the ancestor of Tpx-1 acquired exons 9 and 10 after its divergence from the ancestors of the plant and insect proteins.

Keywords

Sequence Similarity Mouse Chromosome Amino Acid Sequence Similarity Male Germ Cell Computer Search 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1992

Authors and Affiliations

  • Nobuyuki Mizuki
    • 1
  • Donna E. Sarapata
    • 1
  • Jose A. Garcia-Sanz
    • 1
  • Masanori Kasahara
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Miami School of MedicineMiamiUSA

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