Archives of Microbiology

, Volume 160, Issue 4, pp 324–328

Induction of pyruvate decarboxylase in glycolysis mutants of Saccharomyces cerevisiae correlates with the concentrations of three-carbon glycolytic metabolites

  • Eckhard Boles
  • Friedrich K. Zimmermann
Original Papers

DOI: 10.1007/BF00292085

Cite this article as:
Boles, E. & Zimmermann, F.K. Arch. Microbiol. (1993) 160: 324. doi:10.1007/BF00292085


Pyruvate decarboxylase, PDCase, activity in wild-type yeast cells growing on ethanol is quite low but increases up to tenfold upon addition of glucose, less with galactose and only slightly with glycerol. PDCase levels in glycolysis mutant strains growing on ethanol or acetate were higher than in the wild-type strain. These levels correlated with the sum of the concentrations of three-carbon glycolytic metabolites. The highest accumulation was observed in a fructose bisphosphate aldolase deletion mutant concomintant with the highest PDCase activity wild-type level. On the other hand, the PDCase levels in the different mutants again correlated with the sum of the concentrations of the three-carbon glycolytic metabolites. This was interpreted to mean that full induction of PDCase activity requires the accumulation of hexose-and triosephosphates.

Key words

Saccharomyces cerevisiae Pyruvate decarboxylase Pyruvate kinase Signalling Glycolysis mutants 



pyruvate decarboxylase


dry weight





Copyright information

© Springer Verlag 1993

Authors and Affiliations

  • Eckhard Boles
    • 1
  • Friedrich K. Zimmermann
    • 1
  1. 1.Institut für MikrobiologieTechnische HochschuleDarmstadtGermany

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