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Archives of Microbiology

, Volume 160, Issue 4, pp 279–283 | Cite as

Cloning and sequencing of cur A encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174

  • Petra S. Tichaczek
  • Rudi F. Vogel
  • Walter P. Hammes
Original Papers

Abstract

Curvacin A is a bacteriocin produced by Lactobacillus curvatus LTH1174 which is a potential starter organism for the production of fermented dry sausages. This peptide inhibits the growth of the opportunistic food pathogens Listeria monocytogenes and Enterococcus faecalis and thus, curvacin A may enable better performance of a starter and improvement of the hygienic status of meat products. Oligonucleotides were constructed deduced from the peptide sequence and used for the identification of the curvacin A structural gene cur A on a 60 kb plasmid of L. curvatus LTH1174. Plasmidcured derivatives of this strain were unable to produce curvacin A but were still resistant to the bacteriocin. Cur A was cloned into Escherichia coli NM554 and its nucleotide sequence was determined. Sequencing revealed the presence of an additional open reading frame of 51 amino acids with unknown function. A promoter was detected upstream of cur A by primer extension. Both reading frames form a single transcript. Curvacin A is synthesised as a prepeptide of 59 amino acids which is proteolytically processed to the mature bacteriocin of 41 amino acids.

Key words

Bacteriocins Curvacin A Cloning Sequence Lactobacillus curvatus Food hygiene Meal products 

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Copyright information

© Springer Verlag 1993

Authors and Affiliations

  • Petra S. Tichaczek
    • 1
  • Rudi F. Vogel
    • 1
  • Walter P. Hammes
    • 1
  1. 1.Institut für LebensmitteltechnologieUniversität HohenheimStuttgartGermany

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