Human Genetics

, Volume 72, Issue 3, pp 262–265 | Cite as

Assignment of the human homologue of Pim-1, a mouse gene implicated in leukemogenesis, to the pter-q12 region of chromosome 6

  • H. T. Cuypers
  • G. Selten
  • A. Berns
  • A. H. M. Geurts van Kessel
Original Investigations


Viral leukemogenesis in mice is frequently initiated by proviral activation of a highly conserved cellular gene called Pim-1. Here we report the chromosomal localization of the human homologue by Southern blot analyses of DNAs obtained from human-rodent somatic cell hybrids. The single copy human homologue was assigned to the 6pter-q12 segment.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bishop JM, Varmus HE (1982) RNA tumor viruses. In: Weiss RA, Teich N, Varmus HE, Coffin J (eds) The molecular biology of tumor viruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 999–1009Google Scholar
  2. Bodmer WF, Bodmer JG (1978) Evolution and function of the HLA system. Br Med Bull 34:309–316Google Scholar
  3. Corcoran LM, Adams JM, Dunn AR, Cory S (1984) Murine T lymphomas in which the cellular myc oncogene has been activated by retroviral insertion. Cell 37:113–122Google Scholar
  4. Croce CM, Thierfelder W, Erikson J, Nishikura K, Finan J, Lenoir GM, Nowell P (1983) Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a Cλ locus in Burkitt lymphoma cells. Proc Natl Acad Sci USA 80:6922–6926Google Scholar
  5. Cuypers HT, Selten G, Quint W, Zijlsta M, Robanus-Maandag E, Boelens W, van Wezenbeek P, Melief C, Berns A (1984) Murine leukemia virus-induced T-cell lymphomagenesis: integration of proviruses in a distinct chromosomal region. Cell 37:141–150Google Scholar
  6. Fung YKT, Lewis WG, Kung HJ, Crittenden LB (1983) Activation of the cellular oncogene c-erbB by LTR insertion; molecular basis for induction of erythroblastosis by avian leukosis virus. Cell 33:357–368Google Scholar
  7. Geurts van Kessel AHM, ten Brinke H, Boere WAM, den Boer WC, de Groot PG, Hagemeijer A, Meera Khan P, Pearson PL (1981) Characterization of the Philadelphia chromosome by gene mapping. Cytogenet Cell Genet 30:83–91Google Scholar
  8. Geurts van Kessel AHM, Tetteroo PAT, von dem Borne AEGKr, Hagemeijer A, Bootsma D (1983) Expression of human myeloid associated surface antigens in human-mouse myeloid cell hybrids. Proc Natl Acad Sci USA 80:3748–3751Google Scholar
  9. Hayward WS, Neel BG, Astrin S (1981) Activation of a cellular onc gene by promotor insertion in ALV-induced lymphoid leukosis. Nature 290:475–480Google Scholar
  10. Heisterkamp N, Stephenson JR, Groffen J, Hansen PF, de Klein A, Bartram CR, Grosveld G (1983) Localization of the c-able oncogene adjacent to a translocation breakpoint in chronic myelocytic leukemia. Nature 306:239–242Google Scholar
  11. Hilkens J, Cuypers HTh, Selten G, Kroezen V, Hilgers J, Berns A (1986) Genetic mapping of the Pim-1 putative oncogene to mouse chromosome 17. Somatic Cell Mol Genet 12:1–8Google Scholar
  12. Klein G (1983) Specific chromosomal translocations and the genesis of B-cell-derived tumors in mice and man. Cell 32:311–315Google Scholar
  13. Leder P, Battey J, Lenoir G, Moulding C, Murphy W, Potter H, Stewart T, Taub R (1983) Translocation among antibody genes in human cancer. Science 222:765–771Google Scholar
  14. Miyamoto K, Tomita N, Ishii A, Nonaka H, Kondo T, Tanaka T, Kitajima K (1984) Chromosome abnormalities of leukemia cells in adult patients with T-cell leukemia. JNCI 73:353–362Google Scholar
  15. Nadeau JH, Taylor B (1984) Lengths of chromosomal segments conserved since divergence of man and mouse. Proc Natl Acad Sci USA 81:814–818Google Scholar
  16. Neel BG, Hayward WS, Robinson HL, Fang J, Astrin SM (1981) Avian leukosis virus-induced tumors have common proviral integration sites and synthesize discrete new RNAs: oncogenesis by promotor insertion. Cell 23:323–334Google Scholar
  17. Neil JC, Hughes D, McFarlane R, Wilkie NM, Onions DE, Lees G, Jarret O (1984) Transduction and rearrangement of the myc gene by feline leukaemia virus in naturally occurring T-cell leukemias. Nature 308:814–820Google Scholar
  18. Nusse R, Varmus HE (1982) Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell 31:99–109Google Scholar
  19. Payne GS, Bishop JM, Varmus HE (1982) Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas. Nature 295:209–214Google Scholar
  20. Peters G, Brookes S, Smith R, Dickson C (1983) Tumorigenesis by mouse mammary tumor virus: evidence for a common region for provirus integration in mammary tumors. Cell 33:369–377Google Scholar
  21. Robson EB, Lamm LU (1983) Report of the committee on the genetic constitution of chromosome 6. Cytogenet Cell Genet 37:47–70Google Scholar
  22. Selten G, Cuypers HT, Zijlstra M, Melief C, Berns A (1984) Involvement of c-myc in MuLV-induced T cell lymphomas in mice: frequency and mechanisms of activation. EMBO J 3:3215–3222Google Scholar
  23. Selten G, Cuypers HTh, Berns A (1985) Proviral activation of the putative oncogene Pim-1 in MuLV induced T-cell lymphomas. EMBO J 4:1793–1798Google Scholar
  24. Swift RA, Shaller E, Witter RL, Kung HJ (1985) Insertion activation of c-myc by reticuloendotheliosis virus in chicken B lymphoma: nonrandom distribution and orientation of the proviruses. J Virol 54:869–872Google Scholar
  25. Teich N, Wyke J, Mak T, Bernstein A, Hardy W (1982) Pathogenesis of retrovirus-induced disease. In: Weiss RA, Teich N, Varmus HE, Coffin J (eds) RNA tumor viruses. The molecular biology of tumor viruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 785–998Google Scholar
  26. van Someren H, Westerveld A, Hagemeijer A, Mees JR, Meera Khan P, Zaalberg OB (1974) Human antigen and enzyme markers in man-Chinese hamster somatic cell hybrids: evidence for synteny between HL-A, PGMe, ME1 and IPO-B loci. Proc Natl Acad Sci USA 71:962–965Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • H. T. Cuypers
    • 1
  • G. Selten
    • 1
  • A. Berns
    • 1
  • A. H. M. Geurts van Kessel
    • 2
  1. 1.Department of BiochemistryUniversity of NijmegenNijmegenThe Netherlands
  2. 2.Department of Cell Biology and GeneticsErasmus UniversityRotterdamThe Netherlands

Personalised recommendations