Human Genetics

, Volume 69, Issue 4, pp 345–349 | Cite as

Isolation and characterisation of a cDNA clone for human apolipoprotein CI and assignment of the gene to chromosome 19

  • F. Tata
  • I. Henry
  • A. F. Markham
  • S. C. Wallis
  • D. Weil
  • K. H. Grzeschik
  • C. Junien
  • R. Williamson
  • S. E. Humphries
Article

Summary

We have synthesised a mixed oligonucleotide 17 bases long and used it to isolate cDNA clones for apolipoprotein CI (apo CI) from an adult liver cDNA library. The partial sequence of one of these clones confirms its identity. We have used this probe and Southern blotting techniques to identify the human apo CI gene in DNA from a series of rodent x human somatic cell hybrids. Our Results provide evidence for the assignment of this gene to human chromosome 19.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Albers JJ, Lin J, Roberts GP (1979) Effects of human plasma apolipoproteins on the activity of purified lecithin: cholesterol acyl transferase. Artery 5:61–75Google Scholar
  2. Balazs I, Purrello M, Rocchi M, Rinaldi A, Siniscalco M (1982) Is the gene for steroid sulfatase X-linked in man? An appraisal of data from human, mice and their hybrids. Cytogenet Cell Genet 32:251Google Scholar
  3. Barker WC, Dayhoff MO (1977) Evolution of lipoproteins deduced from protein sequence data. Comp Biochem Physiol [B] 57:309–315Google Scholar
  4. Caspersson T, Zech L, Johansson C (1970) Differential binding of alkalating fluorochromes in human chromosomes. Exp Cell Res 60:315–319Google Scholar
  5. Cheung P, Kao FT, Law MT, Jones C, Puck TT, Chan L (1984) Localisation of the structural gene for human apolipoprotein AI on the long arm of chromosome 11. Proc Natl Acad Sci USA 81:508–511Google Scholar
  6. Donald JA, Wallis SC, Kessling A, Tippett P, Robson EB, Ball S, Davies KE, Scambler P, Berg K, Heiberg A, Williamson R, Humphries SE (1985) Linkage relationship of the gene for apolipoprotein CII (Apo CII) with loci on chromosome 19. Hum Genet 69:39–43Google Scholar
  7. Dutrillaux B, Lejeune J (1971) Sur une nouvelle technique d'analyse du caryotype humain. CR Acad Sci (Paris) 272:2638–2640Google Scholar
  8. Herbert PN, Assman G, Gotto AM Jr, Fredrickson DS (1983) In: Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS (eds) The metabolic basis of inherited disease. McGraw-Hill, New York, pp 589–621Google Scholar
  9. Humphries SE, Berg K, Gill L, Cumming AM, Robertson FW, Stalenhoef AFH, Williamson R, Borreson AL (1984) The gene for apolipoprotein CII is closely linked to the gene for apolipoprotein E. Clin Genet 26:389–396Google Scholar
  10. Ish-Horowitz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989–2998Google Scholar
  11. Jackson CL, Bruns GAT, Breslow JL (1984) The isolation and sequence of a human apolipoprotein CII cDNA clone, and its use to isolate and map to human chromosome 19 the human apo CII gene. Proc Natl Acad Sci USA 81:2945–2949Google Scholar
  12. Jeanpierre M, Weil D, Hors-Cayla MC, Williamson R, Junien C, Humphries SE (1984) The gene for apolipoprotein CII is on human chromosome 19. Somatic Cell Mol Genet 10:645–649Google Scholar
  13. Karathanasis SK, McPherson J, Zannis VI, Breslow JL (1983a) Linkage of human apolipoprotein AI and CIII genes. Nature 304:371–373Google Scholar
  14. Karathanasis SK, Zannis VI, Breslow JL (1983b) Isolation and characterisation of the human apolipoprotein AI genes. Proc Natl Acad Sci USA 80:6147–6151Google Scholar
  15. Knott TJ, Robertson ME, Priestley LM, Urdea M, Wallis S, Scott J (1984) Characterisation of mRNAs encoding the precursor for human apolipoprotein CI. Nucleic Acids Res 12:3909–3915Google Scholar
  16. Law SW, Gray G, Brewer HB, Sakaguchi A-Y, Naylor SL (1984) The human apo AI and CIII genes reside in the p11–q13 region of chromosome 11. Biochem Biophys Res Commun 118:934–942Google Scholar
  17. Markham AF, Edge MD, Atkinson TC, Green AR, Heathcliffe GR, Newton CR, Scanlon D (1980) Solid phase phosphotriester synthesis of large oligodeoxy ribonucleotides on a polyamide support. Nucleic Acids Res 8:5193–5205Google Scholar
  18. Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276Google Scholar
  19. Myklebost O, Williamson R, Markham AF, Myklebost S, Rogers J, Woods DE, Humphries SE (1984a) The isolation and characterisation of cDNA clones for human apolipoprotein CII. J Biol Chem 259:4401–4404Google Scholar
  20. Muklebost O, Rogne S, Olaisen B, Gedde-Dahl T Jr, Prydz H (1984b) The locus for apolipoprotein CII is closely linked to the apolipoprotein E locus on chromosome 19 in man. Hum Genet 67:309–312Google Scholar
  21. Olaisen B, Teisberg P, Gedde-Dahl T Jr (1982) The locus for apolipoprotein E (apo E) is linked to the complement component 3 (C3) locus on chromosome 19 in man. Hum Genet 62:233–236Google Scholar
  22. Sanger F, Coulson A, Barrell B, Smith A, Roe B (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161–178Google Scholar
  23. Sanders-Haigh L, Anderson F, Francke U (1980) The β globin gene is on the short arm of human chromosome 11. Nature 283:683–686Google Scholar
  24. Shulman RS, Herbert PN, Wehrly K, Fredrickson DS (1975) The complete amino acid sequence of CI (apo Lp-ser), an apolipoprotein from human very low density lipoproteins. J Biol Chem 280:182–190Google Scholar
  25. Soutar AK, Garner CW, Baker HN, Sparrow JT, Jackson RL, Gotto AM Jr, Smith LC (1975) Effect of the human plasma apolipoproteins and phosphatidylcholine acyl donor on the activity of lecithin: cholesterol acyltransferase. Biochemistry 14:3057–3064Google Scholar
  26. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517Google Scholar
  27. Vieira J, Messing J (1982) The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primer. Gene 19:259–268Google Scholar
  28. Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazo benzylomethyl-paper and rapid hybridisation by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687Google Scholar
  29. Wallis SC, Donald JA, Forrest LA, Williamson R, Humphries SE (1984) The isolation of a genomic clone containing the apoCII gene and the detection of linkage disequilibrium between two common DNA polymorphisms around the gene. Hum Genet 68:286–289Google Scholar
  30. Weil D, Nguyen Van-Cong, Finaz C, Rebourcet R, Cochet C, Grouchy J de, Frezal J (1977) Localisation regionale des genes humains LDHs, MDHs, PGK, αGAL, G6PD par l'hybridation cellulaire interspecifique. Hum Genet 36:205–211Google Scholar
  31. Weil D, Nguyen Van-Cong, Gross MS, Frezal J (1979) Localisation du gene de l'α-glucosidase acide, (α-GLUa) sur le segment q21-qter du chromosome 17 par l'hybridation cellulaire interspecifique. Hum Genet 52:249–257Google Scholar
  32. Weil D, Cottreau D, Nguyen Van-Cong, Rebourcet R, Foubert C, Gross MS, Dreyfus JC, Kahn A (1980) Assignment of the gene for F-type phosphofructokinase to human chromosome 10 by somatic cell hybridisation and specific immunoprecipitation. Ann Hum Genet 44:11–16Google Scholar
  33. Westerveld A, Naylor S (1984) Report of the committee on the genetic constitution of chromosomes 18, 19, 20, 21 and 22. Cytogenet Cell Genet 37:155–175Google Scholar
  34. Woods DE, Markham AF, Richer AT, Goldberger G, Colten HR (1982) Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product. Proc Natl Acad Sci USA 79:5661–5665Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • F. Tata
    • 1
  • I. Henry
    • 2
  • A. F. Markham
    • 3
  • S. C. Wallis
    • 1
  • D. Weil
    • 4
  • K. H. Grzeschik
    • 5
  • C. Junien
    • 2
  • R. Williamson
    • 1
  • S. E. Humphries
    • 1
  1. 1.Department of BiochemistrySt. Mary's Hospital Medical SchoolLondonEngland UK
  2. 2.Groupe de Recherches de Biologie PrenataleINSERM U73, Chateau de LongchampParisFrance
  3. 3.Pharmaceutical DivisionImperial Chemical IndustriesMacclesfieldEngland, UK
  4. 4.Unite de Recherches de Genetique MedicaleINSERM, Centre Hospitalier Universitaire Necker-Enfants MaladeParis Cedex 15France
  5. 5.Institut für Humangenetik der UniversitätMünsterGermany

Personalised recommendations