Human Genetics

, Volume 42, Issue 1, pp 83–88 | Cite as

The effect of SH-SS transition in the structural organization of mitotic chromosomes

  • György Kosztolányi
  • Erica M. Bühler
Original Investigations
Received:

Summary

Human lymphoid cell cultures were treated with 2,2′-dithiodipyridine, a thiol reacting agent which produces SS bridges inside the living cells. After 1 to 4h treatment chromosome preparations were made. The chromosomes acquired a peculiar segmentation along the chromatids. The results suggest that the alteration might arise from a direct reaction of the agent with special chromosomal proteins. The resistance of treated chromosomes to alkali-heat treatment used in C-banding technique was different from that of untreated chromosomes. This prefixational procedure seems to be advantageous in analysing both the chromosome organization and the banding mechanism.

Keywords

Living Cell Thiol Metabolic Disease Structural Organization Lymphoid Cell 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • György Kosztolányi
    • 1
  • Erica M. Bühler
    • 2
  1. 1.Department of PediatricsUniversity of PécsPécsHungary
  2. 2.Department of Genetics, Children's HospitalUniversity of BaselBaselSwitzerland

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