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Human Genetics

, Volume 83, Issue 4, pp 335–338 | Cite as

The gene encoding human low-molecular weight insulin-like growth-factor binding protein (IGF-BP25): regional localization to 7p12–p13 and description of a DNA polymorphism

  • Tiina Alitalo
  • Kimmo Kontula
  • Riitta Koistinen
  • Katriina Aalto-Setälä
  • Mervi Julkunen
  • Olli A. Jänne
  • Markku Seppälä
  • Albert de la Chapelle
Original Investigations

Summary

The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. In situ hybridization studies with a cDNA encompassing the entire protein coding region of IGF-BP25 localized the gene to bands p12–p13 on chromosome 7. Southern blot analysis with the enzyme BglII revealed a common restriction fragment length polymorphism: the presence of the polymorphic BglII site results in the formation of two fragments 4.6 kb and 1.6 kb in size whereas its absence produces a single 6.2 kb fragment. The frequencies of the two alleles were 0.73 and 0.27, respectively. IGF-BP25 constitutes a useful genetic marker for the proximal short arm of chromosome 7.

Keywords

Southern Blot Restriction Fragment Length Polymorphism Southern Blot Analysis Protein Code Region Hybridization Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Tiina Alitalo
    • 1
    • 2
  • Kimmo Kontula
    • 3
  • Riitta Koistinen
    • 4
  • Katriina Aalto-Setälä
    • 3
  • Mervi Julkunen
    • 5
    • 6
  • Olli A. Jänne
    • 5
    • 6
  • Markku Seppälä
    • 4
  • Albert de la Chapelle
    • 1
    • 2
  1. 1.University of HelsinkiDepartment of Medical GeneticsHelsinkiFinland
  2. 2.Folkhälsan Institute of GeneticsHelsinkiFinland
  3. 3.University of HelsinkiInstitute of BiotechnologyHelsinkiFinland
  4. 4.University of HelsinkiDepartment of Obstetrics and GynecologyHelsinkiFinland
  5. 5.The Population CouncilNew YorkUSA
  6. 6.The Rockefeller UniversityNew YorkUSA

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