Human Genetics

, Volume 67, Issue 3, pp 317–325 | Cite as

Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes

  • G. A. Rappold
  • T. Cremer
  • H. D. Hager
  • K. E. Davies
  • C. R. Müller
  • T. Yang
Original Investigations


Two cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization experiments. Our data indicate a large variability of the distances between the sex chromosomes in male and female interphase nuclei. However, the mean distance observed between the X and Y chromosome was significantly smaller than the mean distance observed between the two X-chromosomes. The distribution of distances determined experimentally is compared with three model distributions of distances, and the question of a non-random distribution of sex chromosomes is discussed. Mathematical details of these model distributions are provided in an Appendix to this paper. In the case of a human translocation chromosome (Xqter→Xp22.2::Yq11→Y qter) contained in the Chinese hamster x human hybrid cell line 445 x 393, the binding sites of pXBR and CY1 were found close to each other in most interphase nuclei. These data demonstrate the potential use of chromosome-specific repetitive DNA probes to study the problem of interphase chromosome topography.


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • G. A. Rappold
    • 1
  • T. Cremer
    • 1
  • H. D. Hager
    • 1
  • K. E. Davies
    • 2
  • C. R. Müller
    • 3
  • T. Yang
    • 4
  1. 1.Institut für Anthropologie und HumangenetikUniversität HeidelbergHeidelbergGermany
  2. 2.Biochemistry Department, St Mary's Hospital Medical SchoolUniversity of LondonLondonUK
  3. 3.Institut für HumangenetikUniversität WürzburgWürzburg
  4. 4.Institute of Human GeneticsTexas Medical CenterHoustonUSAGermany

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