Diabetologia

, Volume 28, Issue 11, pp 836–840

The alpha cell response to glucose change during perfusion of anti-insulin serum in pancreas isolated from normal rats

  • H. Maruyama
  • M. Tominaga
  • G. Bolli
  • L. Orci
  • R. H. Unger
Originals

Summary

To determine the effect of neutralization of endogenous insulin upon the glucagon response to a rise and fall of glucose concentration, pancreata isolated from normal rats were perfused with either a potent anti-pork insulin guinea pig serum or a nonimmune guinea pig serum for 30 min. During this period glucose concentration was changed from 100 mg/dl to either 130, 180 or 80 mg/dl for 10 min. Antiserum perfusion at 100 mg/dl caused an approximately two-fold increase in glucagon which was not suppressed by an increase in glucose concentration to either 130 or 180 mg/dl, although glucagon secretion was significantly suppressed in the control experiments in which nonimmune serum was perfused. However, the 0.38±0.21 ng/min rise in glucagon secretion in response to a reduction in glucose concentration to 80 mg/dl in the control experiments was not abolished by antiserum perfusion but, instead, was enhanced (2.66±0.60 ng/min). These findings suggest that insulin may be required for glucose-mediated suppression of glucagon in the isolated pancreas of normal rats but not for stimulation of glucagon secretion by mild glucopenia. Alternatively, neutralization of insulin-mediated release-inhibition of glucagon secretion may simply have altered alpha cell responsiveness in a direction that desensitized it nonspecifically to suppression and sensitized it to stimulation.

Key words

Alpha cells glucagon anti-insulin serum 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • H. Maruyama
    • 1
    • 2
    • 3
  • M. Tominaga
    • 1
    • 2
    • 3
  • G. Bolli
    • 1
    • 2
    • 3
  • L. Orci
    • 1
    • 2
    • 3
  • R. H. Unger
    • 1
    • 2
    • 3
  1. 1.The Center for Diabetes Research of The University of Texas Health Science Center, Veterans Administration Medical CenterDallasUSA
  2. 2.The Institute of Histology and Embryology, University of Geneva School of MedicineGenevaSwitzerland
  3. 3.The University of Perugia School of MedicinePerugiaItaly

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