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Archives of Microbiology

, Volume 156, Issue 2, pp 145–147 | Cite as

Determination of the transmembrane proton gradient in the anaerobic bacterium Desulfovibrio desulfuricans by 31P nuclear magnetic resonance

  • Michael Kroder
  • Peter M. H. Kroneck
  • Heribert Cypionka
Original Papers

Abstract

The transmembrane proton gradient of the sulfate-reducing bacterium Desulfovibrio desulfuricans strain CSN has been determined by in vivo31P nuclear magnetic resonance (NMR) spectroscopy in the absence of dioxygen. At pH 7.0 in the medium (pHex) the intracellular pH (pHin) was 7.5. By lowering pHex to 5.9 pHin decreased to 7.1. At pHex greater than 7.7 the transmembrane proton gradient (ΔpH) was zero. The uncouplers 3,3′,4′,5-tetrachlorosalicylanilide (TCS) and carbonylcyanide-m-chlorophenylhydrazone (CCCP), or the permeant anion thiocyanate caused complete dissipation of ΔpH.

Key words

Transmembrane proton gradient Desulfovibrio desulfuricans CSN 31P NMR Cytoplasmic pH 

Abbreviations

CCCP

carbonylcyanide-m-chlorophenylhydrazone

TCS

3,3′,4′,5-tetrachlorosalicylanilide

MOPS

3-(N-morpholino)-propanesulfonic acid

Pi

inorganic phosphate

pHin (pHex)

intracellular (extracellular) pH

ΔpH

transmembrane proton gradient (pHin-pHex)

ΔΨ

electrochemical membrane potential

δ

chemical shift in parts per million

NMR

nuclear magnetic resonance

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Michael Kroder
    • 1
  • Peter M. H. Kroneck
    • 1
  • Heribert Cypionka
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzGermany

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