Archives of Microbiology

, Volume 159, Issue 4, pp 330–335 | Cite as

Insoluble hydrophobin complexes in the walls of Schizophyllum commune and other filamentous fungi

  • Onno M. H. de Vries
  • M. Peter Fekkes
  • Han A. B. Wösten
  • Joseph G. H. Wessels
Original Papers


Two closely related cysteine-rich hydrophobic proteins, Sc3p and Sc4p, of the basidiomycete Schizophyllum commune are developmentally regulated and associated with the walls of aerial hyphae and fruit-body hyphae. They are present in the walls as hot-SDS-insoluble complexes which can be extracted with formic acid. The hydrophobins can then be dissociated by oxidation with performic acid. However, extraction of the walls with trifluoroacetic acid results in both solubilization and dissociation of the hydrophobin complexes into monomers. This suggests that non-covalent interactions are responsible for formation of these insoluble complexes. Carboxymethylation with iodoacetic acid only occurred after reduction with DTT indicating all cysteines in the monomeric hydrophobins involved in intramolecular disulfide bridges. Abundant proteins with similar properties were found in walls from all other filamentous fungi tested, including the basidiomycetes Pleurotus ostreatus, Coprinus cinereus, Agaricus bisporus, and Phanerochaete chrysosporium, the ascomycetes Aspergillus nidulans, Neurospora crassa, and Penicillium chrysogenum, and the zygomycete Mucor mucedo.

Key words

Hydrophobins Cell walls Schizophyllum commune hydrophobins in Filamentous fungi hydrophobins in Trifluoroacetic acid 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Onno M. H. de Vries
    • 1
  • M. Peter Fekkes
    • 1
  • Han A. B. Wösten
    • 1
  • Joseph G. H. Wessels
    • 1
  1. 1.Department of Plant Biology, Biological CenterUniversity of GroningenHarenThe Netherlands

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