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Molecular and General Genetics MGG

, Volume 246, Issue 6, pp 739–744 | Cite as

RNA editing of a group II intron in Oenothera as a prerequisite for splicing

  • G. Valentin Börner
  • Mario Mörl
  • Bernd Wissinger
  • Axel Brennicke
  • Carlo Schmelzer
Original Paper

Abstract

The trans-splicing group II intron c/d in the Oenothera mitochondrial nad1 gene is modified by RNA editing in domain 6. This C-to-U conversion generates the typical domain 6 structure, which prompted us to speculate that this RNA editing event might be essential for splicing. To test this hypothesis, we investigated the influence of unedited and edited sequences of the Oenothera intron on splicing in vitro. The stem of domain 6 of intron nad1-c/d was transplanted into the autocatalytic yeast intron aI5c, yielding chimeras with the genomic C and the edited U, respectively, 5′ of the branchpoint A. When incubated under self-splicing conditions, only the edited chimera was released as a lariat, while the precursor with the genomically coded C remained inactive. Our results support the hypothesis that Oenothera group II intron nadl-c/d cannot be spliced from the primary transcript without previous editing in domain 6.

Key words

Group II intron Plant mitochondria Regulation of splicing RNA editing 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • G. Valentin Börner
    • 1
  • Mario Mörl
    • 1
  • Bernd Wissinger
    • 2
  • Axel Brennicke
    • 2
  • Carlo Schmelzer
    • 1
  1. 1.Institut für Genetik und MikrobiologieUniversität MünchenMünchenGermany
  2. 2.Institut für Genbiologische ForschungBerlinGermany

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