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Molecular and General Genetics MGG

, Volume 230, Issue 1–2, pp 39–44 | Cite as

Cloning and expression analysis of a potato cDNA that encodes branching enzyme evidence for co-expression of starch biosynthetic genes

  • Jens Koßmann
  • Richard G. F. Visser
  • Bernd Müller-Röber
  • Lothar Willmitzer
  • Uwe Sonnewald
Article

Summary

One of the key enzymes involved in the formation of amylopectin, which is the major component of starch, is branching enzyme. A cDNA for potato branching enzyme was cloned by screening a tuber-specific cDNA expression library using an antiserum directed against a denatured preparation of the protein. Complementation of an Escherichia coli strain deficient in branching enzyme was achieved using a construct derived from this clone. Analysis of the expression of the gene in potato revealed a close association with conditions favouring starch biosynthesis. The expression pattern of the gene coding for potato branching enzyme, as analyzed at the mRNA level, closely resembles that of 4GPaseS, a gene coding for one of the subunits of ADP-glucose pyrophosphorylase, which is the key regulatory enzyme in the starch biosynthetic pathway. This raises the possibility that enzymes involved in the pathway are coordinately regulated at the transcriptional level.

Key words

Branching enzyme Potato Starch synthesis Sucrose induction 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Jens Koßmann
    • 1
  • Richard G. F. Visser
    • 2
  • Bernd Müller-Röber
    • 1
  • Lothar Willmitzer
    • 1
  • Uwe Sonnewald
    • 1
  1. 1.Institut für Genbiologische Forschung Berlin GmbHBerlin 33Germany
  2. 2.Plant Breeding Institute (IvP)Agricultural UniversityWageningenThe Netherlands

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