Molecular and General Genetics MGG

, Volume 249, Issue 3, pp 274–280 | Cite as

Cloning and nucleotide sequence of fosfomycin biosynthetic genes of Streptomyces wedmorensis

  • Tomomi Hidaka
  • Masao Goda
  • Tomohisa Kuzuyama
  • Naomi Takei
  • Makoto Hidaka
  • Haruo Seto
Original Paper


The biosynthetic pathway for production of the antibiotic fosfomycin by Streptomyces wedmorensis consists of four steps including the formation of a C-P bond and an epoxide. Fosfomycin production genes were cloned from genomic DNA using S. wedmorensis mutants blocked at different steps of the biosynthetic pathway. Four genes corresponding to each of the biosynthetic steps were found to be clustered in a DNA fragment of about 5 kb. Nucleotide sequencing of a large fragment revealed the presence of ten open reading frames, including the four biosynthetic genes and six genes with unknown functions.

Key words

Fosfomycin C-P bond 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Tomomi Hidaka
    • 1
    • 2
  • Masao Goda
    • 1
  • Tomohisa Kuzuyama
    • 1
  • Naomi Takei
    • 1
  • Makoto Hidaka
    • 1
  • Haruo Seto
    • 1
  1. 1.Institute of Molecular and Cellular BiosciencesThe University of TokyoBunkyo-ku, TokyoJapan
  2. 2.Department of Biotechnology, Division of Agriculture and Agricultural Life SciencesThe University of TokyoBunkyo-ku, TokyoJapan

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