Molecular and General Genetics MGG

, Volume 249, Issue 1, pp 74–81

A high-resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markers

  • Khalid Meksem
  • Dario Leister
  • Johan Peleman
  • Marc Zabeau
  • Francesco Salamini
  • Christiane Gebhardt
Original Paper

Abstract

The R1 allele confers on potato a race-specific resistance to Phytophthora infestans. The corresponding genetic locus maps on chromosome V in a region in which several other resistance genes are also located. As part of a strategy for cloning R1, a high-resolution genetic map was constructed for the segment of chromosome V that is bordered by the RFLP loci GP21 and GP179 and includes the R1 locus. Bulked segregant analysis and markers based on amplified fragment length polymorphisms (AFLP markers) were used to select molecular markers closely linked to R1. Twenty-nine of approximately 3200 informative AFLP loci displayed linkage to the R1 locus. Based on the genotypic analysis of 461 gametes, eight loci mapped within the GP21–GP179 interval. Two of those could not be seperated from R1 by recombination. For genotyping large numbers of plants with respect to the flanking markers GP21 and GP179 PCR based assays were also developed which allowed marker-assisted selection of plants with genotypes Rr and rr and of recombinant plants.

Key words

Potato Phytophthora infestans Resistance Map-based cloning AFLP markers 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Khalid Meksem
    • 1
  • Dario Leister
    • 1
  • Johan Peleman
    • 2
  • Marc Zabeau
    • 2
  • Francesco Salamini
    • 1
  • Christiane Gebhardt
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKölnGermany
  2. 2.KeyGeneAE WageningenThe Netherlands

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