Molecular and General Genetics MGG

, Volume 246, Issue 1, pp 56–64

Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants

  • Arunik Sanyal
  • Alexis Harington
  • Christopher J. Herbert
  • Olga Groudinsky
  • Piotr P. Slonimski
  • Beatrice Tung
  • Godfrey S. Getz
Original Paper

Abstract

mna2, which belongs to the class I temperature-sensitive pet mutants that lose mitochondrial (mt)RNA at restrictive temperature, was shown by complementation and sequence determination to correspond to the gene coding for HSP60. Both mna2-1 and mna2-2, the two available alleles of mna2, have conservative single amino acid substitutions in the HSP60 gene. Valine substitutes for an alanine (position 47) in mna2-1, and an isoleucine substitutes for a valine (position 77) in mna2-2. These substitutions result in defects in respiration and in steady-state mtRNA accumulation. Wild-type hsp60 alleviates the mtRNA phenotype completely, while partially relieving the respiratory deficiency.

Key words

Temperature-sensitive mutants Heat shock protein 60 Conservative single amino acid substitutions Mitochondrial biogenesis Saccharomyces cerevisiae 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Arunik Sanyal
    • 1
  • Alexis Harington
    • 2
  • Christopher J. Herbert
    • 2
  • Olga Groudinsky
    • 2
  • Piotr P. Slonimski
    • 2
  • Beatrice Tung
    • 3
  • Godfrey S. Getz
    • 1
    • 3
    • 4
  1. 1.Department of MedicineUniversity of ChicagoUSA
  2. 2.Centre de Genetique Moleculaire du C.N.R.S.Laboratoire propre associé a' l'Université Pierre et Marie CurieGif-sur-YvetteFrance
  3. 3.Department of PathologyUniversity of ChicagoChicagoUSA
  4. 4.Department of Biochemistry and Molecular BiologyUniversity of ChicagoChicagoUSA

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