Summary
Pollen embryogenesis and subsequent plant regeneration have been established from cultured whole barley spikes in agitated N6 liquid medium (Chu 1978) containing high levels of 2,4-D, Ficoll and potato extract. Microspore division within the anthers and subsequent embryogenic development were obtained in medium containing high amounts of reduced nitrogen with Zeatin, NAA and BAP (all at 0.5 mg/l levels, pH 6.2). Once embryoids were formed in the liquid medium, they produced secondary embryoids from the scutellum and subsequently plants on MS (Murashige and Skoog 1962) agar medium containing BAP and NAA. The ratio of green plants to albino was 1∶8.7.
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References
Clapham D (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzücht 69:142–145
Cheng TY, Smith HH (1975) Organogenesis from callus cultures of Hordeum vulgare. Planta 123:307–310
Chu CC (1978) The N6 medium and its application to anther culture of cereal crops. In: Proc Symp Plant Tissue Culture. Science Press, Beijing, China, pp 43–50
Dale PJ, Deambrogio E (1979) A comparison of callus induction and plant regeneration from different expiants of Hordeum vulgare. Z Pflanzenphysiol 94:65–77
Dunwell JM (1985) Anther and ovary culture. In: Bright SWJ, Jones MGK (eds) Cereal cell and tissue culture. Nijhoff/Junk, Dordrecht, pp 1–44
Datta SK, Wenzel G (1987) Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L. Plant Sci 48:49–54
Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9:671–678
Foroughi-Wehr B, Mix G, Gaul H, Wilson HM (1976) Plant production from cultured anthers of Hordeum vulgare L. Z Pflanzenzucht 77:198–204
Goldstein CS, Kronstad WE (1986) Tissue culture and plant regeneration from immature embryo expiants of barley, Hordeum vulgare. Theor Appl Genet 71:631–636
Han H (1985) Use of haploids for crop improvement in China. GMICN 1:11–23
Hanzel JJ, Miller JP, Brinkman MA, Fendos E (1985) Genotypes and media effects on callus formation and regeneration in barley. Crop Sci 25:27–31
Heberle-Bors E (1985) In vitro haploid formation from pollen: a critical review. Theor Appl Genet 71:361–374
Huang B, Sunderland N (1982) Temperature stress pretreatment in barley anther culture. Ann Bot 49:77–88
Kao KN (1981) Plant formation from Barley anther culture with Ficoll media. Z Pflanzenphysiol 103:437–443
Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876
Köhler F, Wenzel G (1985) Regeneration of isolated barley microspores in conditioned media and trials to characterise the responsible factor. J Plant Physiol 121:181–191
Lörz H, Brown P, Göbel E, Junker B, Lührs R, Stolarz A, Zimmy J (1986) In vitro manipulation of cereal crops. In: Semal J (ed) Somaclonal variation and crop improvement. Proc CEC Seminar. Martinus Nijhoff, Dordrecht Boston Lancaster, pp 117–126
Miah MAA, Earle ED, Khush GS (1985) Inheritance of callus formation ability in anther culture of rice, Oryza sativa L. Theor Appl Genet 70:113–116
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–479
Potrykus I, Harms CT, Lörz H (1979) Callus formation and cell culture protoplasts of corn (Zea mays). Theor Appl Genet 54:209–214
Sunderland N, Huang B (1985) Barley anther culture — the switch of programme and albinism. Hereditas (Suppl) 3:27–40
Vasil IK (1983) Regeneration of plants from single cells of cereals and grasses. In: Lurquin PF, Kleinhofs A (eds) Genetic engineering in eukaryotes. Plenum, New York, pp 233–252
Wei ZM, Kyo M, Harada H (1986) Callus formation and plant regeneration through direct culture of isolated pollen of Hordeum vulgare cv. ‘Sabarlis’. Theor Appl Genet 72:252–255
Weigel RC, Hughes KW (1985) Longterm regeneration by somatic embryogenesis in barley (Hordeum vulgare) tissue cultures derived fom apical meristem expiants. Plant Cell Tissue Organ Cult 5:151–162
Wenzel G, Foroughi-Wehr B, Friedt W, Köhler F, Oo T (1985) Cell and tissue culture as supplementary tool in plant breeding: exemplified in potato, oilseed rape and barley. Hereditas (Suppl) 3:15–25
Wilson HM (1977) Culture of whole barley spikes stimultes high frequencies of pollen calluses in individual anthers. Plant Sci Lett 9:233–238
Xu ZH, Huang B, Sunderland N (1981) Culture of barley anthers in conditioned media. J Exp Bot 32:767–778
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Datta, S.K. Plant regeneration by pollen embryogenesis from cultured whole spikes of barley (Hordeum vulgare). Theoret. Appl. Genetics 74, 121–124 (1987). https://doi.org/10.1007/BF00290094
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DOI: https://doi.org/10.1007/BF00290094