Theoretical and Applied Genetics

, Volume 75, Issue 4, pp 628–641

Linkage mapping of genes controlling endosperm storage proteins in wheat

1. Genes on the short arms of group 1 chromosomes
  • N. K. Singh
  • K. W. Shepherd
Originals

Summary

A translocation mapping procedure was used to map gene-centromere distances for the genes controlling endosperm proteins on the short arm of each of the chromosomes 1A, 1B and 1D in wheat. The genes controlling triplet proteins (tentatively designated Tri-1) were found to be closely linked to the centromere on chromosome arms 1AS and 1DS and loosely linked to the gliadin genes (Gli-1) on the same arms. The Gli-1 genes segregated independently or were very loosely linked to their respective centromeres. The Gli-B1-centromere map distance on 1BS was also estimated using conventional telocentric mapping and the result was similar to that obtained with the translocation mapping. A simple two-step one-dimensional electrophoretic procedure is described which allows the low-molecular-weight (LMW) glutenin subunits to be separated from the gliadin bands, thus facilitating the genetic analysis of these LMW subunits. No recombination was observed between the genes (designated Glu-3) controlling some major LMW glutenin subunits and those controlling gliadins on chromosome arms 1AS and 1DS. However, in a separate experiment, the genes controlling LMW glutenin subunits on 1BS (Glu-B3) showed a low frequency of recombination with the gliadin genes.

Key words

Wheat Triplet proteins Gliadins Glutenins Linkage mapping 

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References

  1. Chojecki AJS, Gale MD, Holt LM, Payne PI (1983) The intrachromosomal mapping of a glucose phosphate isomerase structural gene, using allelic variation among stocks of Chinese Spring wheat. Genet Res 41:221–226Google Scholar
  2. Driscoll CJ, Sears ER (1971) Individual addition of the chromosomes of‘Imperial’ rye to wheat. Agron Abstr, p 6Google Scholar
  3. Fu JK, Sears ER (1973) The relationship between chiasmata and crossing over in Triticum aestivum. Genetics 75:231–246Google Scholar
  4. Galili G, Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193:293–298Google Scholar
  5. Hanson WD (1959) Minimum family sizes for the planning of genetic experiments. Agronomy 151:711–716Google Scholar
  6. Jackson EA, Holt LM, Payne PI (1983) Characterization of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal location of their controlling genes. Theor Appl Genet 66:29–37Google Scholar
  7. Jackson EA, Holt LM, Payne PI (1985) Glu-B2, a storage protein locus controlling the D group of LMW glutenin subunits in bread wheat (Triticum aestivum). Genet Res 46:11–17Google Scholar
  8. Koebner RMD, Shepherd KW (1986) Controlled introgression to wheat of genes from rye chromosome arm 1RS by induction of allosyndesis. 1. Isolation of recombinants. Theor Appl Genet 73:197–208Google Scholar
  9. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  10. Lawrence GJ, Shepherd KW (1981a) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31Google Scholar
  11. Lawrence GJ, Shepherd KW (1981b) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337Google Scholar
  12. Mather K (1951) The measurement of linkage in heredity. Methuen's Monographs on Biological Subjects, 2nd edn. LondonGoogle Scholar
  13. Metakovsky EV, Novoselskaya AYu, Kopus MM, Sobko TA, Sozinov AA (1984) Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 67:559–568Google Scholar
  14. Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35Google Scholar
  15. Payne PI, Holt LM, Worland AT, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. 3. Telocentric mapping of the subunit genes on the long arms of homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138Google Scholar
  16. Payne PI, Jackson EA, Holt LM, Law CN (1984a) Genetic linkage between endosperm protein genes on each of the short arms of chromosomes 1A and 1B in wheat. Theor Appl Genet 67:235–243Google Scholar
  17. Payne PI, Holt LM, Hutchinson J, Bennett MD (1984b) Development and characterisation of a line of bread wheat, Triticum aestivum, which lacks the short-arm satellite of chromosome 1B and the Gli-B1 locus. Theor Appl Genet 68:327–334Google Scholar
  18. Rybalka AI, Sozinov AA (1978) Mapping the locus of Gld-1B, which controls the biosynthesis of reserve proteins in soft wheat. Tsitol Genet 13:276–282Google Scholar
  19. Sears ER (1954) The aneuploids of common wheat. Mo Agric Exp Stn Res Bull 572:1–58Google Scholar
  20. Sears ER (1962) The use of telocentrics in linkage mapping. Genetics 47:983Google Scholar
  21. Sears ER (1966) Chromosome mapping with the aid of telocentrics. In: Proc 2nd Int Wheat Genet Symp. Lund, Sweden. Hereditas (Suppl) 2:370–381Google Scholar
  22. Sears ER, Sears LMS (1978) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proc 5th Int Wheat Genet Symp. Indian Soc Genet Plant Breed, New Delhi, pp 389–407Google Scholar
  23. Sears ER, Loegering WQ, Rodenhiser HA (1957) Identification of chromosomes carrying genes for stem rust resistance in four varieties of wheat. Agronomy J 49:208–212Google Scholar
  24. Shepherd KW (1968) Chromosomal control of endosperm proteins in wheat and rye. In: Finlay KW, Shepherd KW (eds) Proc 3rd Int Wheat Genet Symp. Aust Acad Sci, Canberra, pp 86–96Google Scholar
  25. Shepherd KW (1973) Homoeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, pp 745–760Google Scholar
  26. Shewry PR, Bradberry D, Franklin J, White RP (1984) The chromosomal locations and linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Theor Appl Genet 69:63–69Google Scholar
  27. Shewry PR, Miflin BJ (1985) Seed storage proteins of economically important cereals. In: Pomeranz Y (ed) Adv Cereal Sci Technol, vol VII. St Paul, Minnesota, pp 1–84Google Scholar
  28. Singh NK, Shepherd KW (1984a) Mapping of the genes controlling high-molecular-weight glutelin subunits of rye on the long arm of chromosome 1R. Genet Res 44:117–123Google Scholar
  29. Singh NK, Shepherd KW (1984b) A new approach to studying the variation and genetic control of disulphide-linked endosperm proteins in wheat and rye. In: Graveland A, Moonen JHE (eds) Proc 2nd Int Workshop Gluten Proteins. Wageningen, pp 129–136Google Scholar
  30. Singh NK, Shepherd KW (1985) The structure and genetic control of a new class of disulphide-linked proteins in wheat endosperm. Theor Appl Genet 71:79–92Google Scholar
  31. Singh NK, Shepherd KW (1987) Solubility behaviour, synthesis, degradation and subcellular location of a new class of disulphide-linked proteins in wheat endosperm. Aust J Plant Physiol 14:245–252Google Scholar
  32. Singh NK, Shepherd KW, Langridge P (1986) Characterization of triplet proteins-globulin type proteins present in wheat gluten. In: Murray L (ed) Proc 36th Aust Cereal Chem Conf Adelaide, Australia, pp 86–90Google Scholar
  33. Sozinov AA, Poperelya FA (1980) Genetic classification of prolamines and its use for plant breeding. Ann Technol Agric 29:229–245Google Scholar
  34. Vosa CG, Marchi P (1972) Quinacrine fluorescence and Giemsa staining in plants. Nature (New Biol) 237:191–192Google Scholar
  35. Wrigley CW, Shepherd KW (1973) Electrofocusing of grain proteins from wheat genotypes. Ann NY Acad Sci 209:154–162Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • N. K. Singh
    • 1
  • K. W. Shepherd
    • 1
  1. 1.Agronomy DepartmentWaite Agricultural Research Institute, The University of AdelaideGlen OsmondAustralia

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