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Human Genetics

, Volume 51, Issue 2, pp 183–194 | Cite as

Transplacental mutagenesis: The micronucleus test on fetal mouse blood

  • M. -T. King
  • D. Wild
Original Investigations

Summary

The induction of cytogenetic damage (micronuclei) in mouse fetal blood was studied with four selected mutagens: cyclophosphamide, procarbazine, trenimon, and mitomycin-C. For comparison the standard micronucleus test on maternal bone marrow was also performed. In contrast to the results obtained from maternal bone marrow the changes in the cellular composition in fetal blood were only slight after treatment with mutagens. A significant and dosepdependent increase in the incidence of micronucleated fetal blood cells was found with all four mutagens. The inducibility of micronuclei by indirect mutagens was particularly interesting. The three mutagens other than mitomycin-C induced a higher frequency of micronucleated polychromatic erythrocytes in fetal blood cells than in maternal bone marrow. The results indicate that this modified micronucleus test is well suited and useful for mutagenicity screening of environmental chemicals and especially for assessment of risks to the fetus when pregnant females are exposed to environmental chemicals.

Keywords

Internal Medicine Cyclophosphamide Metabolic Disease Environmental Chemical Mutagenicity Screening 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • M. -T. King
    • 1
  • D. Wild
    • 1
  1. 1.Zentrallaboratorium für MutagenitätsprüfungFreiburgFederal Republic of Germany

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