Human Genetics

, Volume 83, Issue 2, pp 159–164

Chromosomal localization of human aspartate aminotransferase genes by in situ hybridization

  • S. Pol
  • B. Bousquet-Lemercier
  • M. Pavé-Preux
  • F. Bulle
  • E. Passage
  • J. Hanoune
  • M. G. Mattei
  • R. Barouki
Original Investigations

DOI: 10.1007/BF00286710

Cite this article as:
Pol, S., Bousquet-Lemercier, B., Pavé-Preux, M. et al. Hum Genet (1989) 83: 159. doi:10.1007/BF00286710

Summary

The localization of the human genes for cytosolic and mitochondrial aspartate aminotransferase (AspAT) has been determined by chromosomal in situ hybridization with specific human cDNA probes previously characterized in our laboratory. The cytosolic AspAT gene is localized on chromosome 10 at the interface of bands q241–q251. Mitochondrial AspAT is characterized by a multigene family located on chromosomes 12 (p131–p132), 16 (q21), and 1 (p32–p33 and q25–q31). Genomic DNA from ten blood donors was digested by ten restriction enzymes, and Southern blots were hybridized with the two specific probes. Restriction fragment length polymorphism was revealed in only one case for cytosolic AspAT, with PvuII, while no polymorphism for mitochondrial AspAT was found.

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • S. Pol
    • 1
  • B. Bousquet-Lemercier
    • 1
  • M. Pavé-Preux
    • 1
  • F. Bulle
    • 1
  • E. Passage
    • 2
  • J. Hanoune
    • 1
  • M. G. Mattei
    • 2
  • R. Barouki
    • 1
  1. 1.Institut National de la Santé et de la Recherche Médicale (INSERM)Hôpital Henri MondorCréteilFrance
  2. 2.Institut National de la Santé et de la Recherche MédicaleHôpital des Enfants de la TimoneMarseille Cédex 5France

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