Human Genetics

, Volume 65, Issue 2, pp 144–146 | Cite as

Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands

  • T. A. Donlon
  • R. E. Magenis
Original Investigations


The DA/DAPI technique has been found to be useful in the identification of specific chromosomal regions on human chromosomes. The realization that distamycin A (DA) is no longer commercially available has necessitated the development of an alternative technique. We describe a technique, MG/DAPI, which substitutes the AT-specific dye methyl green for distamycin A and gives results identical to those of the DA/DAPI technique.


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  1. Arrighi FE, Hsu TC (1971) Localization of heterochromatin in human chromosomes. Cytogenetics 10: 81–86Google Scholar
  2. Behe M, Felsenfeld G (1981) Effects of methylation on a synthetic polynucleotide: The B-Z transition in poly(dG-m5dC)·poly (dG-m5dC). Proc Natl Acad Sci USA 78: 1619–1623Google Scholar
  3. Bobrow M, Madan K, Pearson PL (1972) Staining of some specific regions of human chromosomes, particularly the secondary constriction of no.9. Nature 238: 122–124Google Scholar
  4. Donlon TA (1981) Cytologic characterization of human constitutive heterochromatin. Master of Science Thesis, Portland State UniversityGoogle Scholar
  5. Gagné R, Laberge C (1972) Specific cytological recognition of the heterochromatic segment of number 9 chromosome in man. Exp Cell Res 73: 239–242Google Scholar
  6. Kurnick NB, Foster M (1980) Methyl green. III. Reaction with deoxyribonucleic acid, stoichiometry, and behavior of the reaction product. J Gen Physiol 34: 147–159Google Scholar
  7. Latt SA, Juergenss LA, Mathews DJ, Gustashaw KW, Sahar E (1981) Energy-enhanced chromosome banding. An overview. Cancer Genet Cytogenet 1: 187–196Google Scholar
  8. Lin MS, Comings DE, Alfi OS (1977) Optical studies of the interaction of 4′-6-diamidino-2-phenylindole with DNA and metaphase chromosomes. Chromosoma 60: 15–25Google Scholar
  9. Magenis E, Donlon T (1982) Nonfluorescent Y chromosomes: cytologic evidence of origin. Hum Genet 60: 133–138Google Scholar
  10. Magenis E, Brown MG, Chamberlin J, Donlon T, Hepburn D, Lamvik N, Lovrien E, Yoshitomi M (1981) Resolution of breakpoints in a complex rearrangement by use of multiple staining techniques: confirmation of suspected 12p12.3 intraband deletion dosage effect of LDHB. Am J Med Genet 9: 95–103Google Scholar
  11. Miller OJ, Schnedl W, Allen J, Erlanger BF (1974) 5-Methylcytosine localized in mammalian constitutive heterochromatin. Nature 251: 636–637Google Scholar
  12. Müller W, Gautier F (1975) Interactions of heteroaromatic compounds with nucleic acids. AT-specific non-intercalating DNA ligands. Eur J Biochem 54: 385–394Google Scholar
  13. Sahar E, Latt SA (1980) Energy transfer and binding competition between dyes used to enhance staining differentiation in metaphase chromosomes. Chromosoma 79: 1–28Google Scholar
  14. Schnedl W, Abraham R, Dann O, Geber G, Schweizer D (1981) Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15 and the Y by D 287/170. Hum Genet 59: 10–13Google Scholar
  15. Schreck RR, Dev VG, Erlanger BF, Miller OJ (1977) The structural organization of mouse metaphase chromosomes. Chromosoma 62: 337–350Google Scholar
  16. Schweizer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111: 327–332Google Scholar
  17. Wisniewski L, Hassold T, Hefflinger J, Higgins J (1979) Cytogenetic and clinical studies in five cases of inv dup(15). Hum Genet 50: 259–270Google Scholar
  18. Zimmer C (1975) Effects of the antibiotic netropsin and distamycin A on the structure and function of nucleic acids. Prog Nucleic Acid Res Mol Biol 15: 285–318Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • T. A. Donlon
    • 1
  • R. E. Magenis
    • 1
  1. 1.Department of Medical Genetics and the Child Development and Rehabilitation CenterOregon Health Sciences UniversityPortlandUSA

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