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Human Genetics

, Volume 66, Issue 2–3, pp 267–271 | Cite as

Complete pachytene chromomere karyotypes of human spermatocyte bivalents

  • J. M. Luciani
  • M. R. Guichaoua
  • M. R. Morazzani
Original Investigations

Summary

Well-spread human pachytene spermatocyte bivalents were obtained allowing specific identification of each bivalent within its total complement according to its chromomere sequence combined with further staining of its centromeric heterochromatin. The total number of chromomeres was found to be related to the degree of bivalent contraction: 396 in condensed bivalents and 511 in decondensed bivalents. A striking correspondance between chromomeres and mitotic G-bands was observed; on account of the variability of bivalent contraction, condensed bivalents corresponded to prometaphase somatic chromosomes and decondensed bivalents to mid/late prophase chromosomes.

Keywords

Internal Medicine Metabolic Disease Specific Identification Somatic Chromosome Pachytene Spermatocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Francke U, Oliver N (1978) Quantitative analysis of high resolution trypsin-Giemsa bands of human prometaphase chromosomes. Hum Genet 45:137–165Google Scholar
  2. Hungerford DA, Hungerford AM (1978) Chromosome structure and function. IV. Pachytene chromomere maps of 16, 17 and 18; pachytene as a reference standard for metaphase banding. Cytogenet Cell Genet 21:212–230Google Scholar
  3. Hungerford DA, La Badie GU, Balaban GB (1971) Chromosome structure and function in man. II. Provisional maps of the two smallest autosomes (chromosome 21 and 22) at pachytene in the male. Cytogenetics 10:33–37Google Scholar
  4. ISCN (1981) An international system for human cytogenetic nomenclature: high resolution banding. Cytogenet Cell Genet 31:5–18Google Scholar
  5. Jagiello GM, Fang JS (1982) Complete autosomal chromomere maps of human early and mid/late pachytene spermatocytes. Am J Hum Genet 34:112–124Google Scholar
  6. Jhanwar SC, Burns JP, Alonso ML, Hew W, Chaganti RSK (1982) Mid-pachytene chromomere maps of human autosomes. Cytogenet Cell Genet 33:240–248Google Scholar
  7. Luciani JM, Morazzani MR, Stahl A (1975) Identification of pachytene bivalents in human male meiosis using G-banding technique. Chromosoma 52:275–282Google Scholar
  8. Luciani JM, Devictor M, Morazzani MR, Stahl A (1976) Meiosis of trisomy 21 in the human pachytene oocyte. Chromosoma 57: 155–163Google Scholar
  9. Luciani JM, Devictor M, Morazzani MR, Stahl A (1977) Pachytene mapping of the C9 and acrocentric bivalents in the human oocyte. Hum Genet 36:197–204Google Scholar
  10. Luciani JM, Devictor M, Boue J, Morazzani MR, Boue A, Stahl A (1978) Etude de la méiose ovocytaire chez un foetus trisomique 18. Comportement du chromosome surnuméraire et identification du bivalent 18. Ann Génét (Paris) 21:215–218Google Scholar
  11. Oud JL, Reutlinger AHH (1981) Chromosome behaviour during early meiotic prophase of mouse primary spermatocytes. Chromosoma 83:395–407Google Scholar
  12. Solari AJ (1980) Synaptonemal complexes and associated structures in microspread human spermatocytes. Chromosoma 81:315–337Google Scholar
  13. Yunis JJ (1981) Mid-prophase human chromosomes. The attainment of 2000 bands. Hum Genet 56:293–298Google Scholar
  14. Yunis JJ, Sawyer JR, Ball DW (1978) The characterization of high resolution G-banded chromosomes of man. Chromosoma 67: 293–307Google Scholar
  15. Yunis JJ, Ball DW, Sawyer JR (1979) G-banding patterns of high resolution human chromosomes 6-22, X and Y. Hum Genet 49:291–306Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. M. Luciani
    • 1
  • M. R. Guichaoua
    • 1
  • M. R. Morazzani
    • 1
  1. 1.Department of Embryology and CytogeneticsFaculty of MedicineMarseillesFrance

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