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Human Genetics

, Volume 65, Issue 3, pp 257–261 | Cite as

Chromosome assignment of two cloned DNA probes hybridizing predominantly to human sex chromosomes

  • Gudrun A. Rappold
  • T. Cremer
  • C. Cremer
  • W. Back
  • J. Bogenberger
  • H. J. Cooke
Original Investigations

Summary

In situ hybridization experiments were carried out with two clones, YACG 35 and 2.8, which had been selected from two genomic libraries strongly enriched for the human Y chromosome. Besides the human Y chromosome, both sequences strongly hybridized to the human X chromosome, with few minor binding sites on autosomes. In particular, on the X chromosome DNA from clone YACG 35 hybridized to the centromeric region and the distal part of the short arm (Xp2.2). On the Y chromosome, the sequence was assigned to one site situated in the border region between Yq1.1 and Yq1.2. DNA from clone 2.8 also hybridized to the centromeric region of the X and the distal part of the short arm (Xq2.2). On the Y, however, two binding sites were observed (Yp1.1 and Yq1.2). The findings indicate that sex chromosomal sequences may be localized in homologous regions (as suggested from meiotic pairing) but also at ectopic sites.

Keywords

Internal Medicine Metabolic Disease Distal Part Genomic Library Hybridization Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Gudrun A. Rappold
    • 1
  • T. Cremer
    • 1
  • C. Cremer
    • 2
  • W. Back
    • 1
  • J. Bogenberger
    • 3
  • H. J. Cooke
    • 4
  1. 1.Institut für Anthropologie und HumangenetikUniversität HeidelbergHeidelbergFederal Republic of Germany
  2. 2.Institut für Angewandte PhysikUniversität HeidelbergHeidelbergFederal Republic of Germany
  3. 3.Institut für Physiologische ChemieUniversität MünchenMünchen 2Federal Republic of Germany
  4. 4.MRC Mammalian Genome Unit, Department of ZoologyUniversity of EdinburghGB-Edinburgh

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