Advertisement

Chromosoma

, Volume 82, Issue 2, pp 217–227 | Cite as

The link between dosage compensation and sex differentiation in Drosophila melanogaster

  • John C. Lucchesi
  • Thomas Skripsky
Article

Abstract

The rate of 3H-uridine incorporation into X-chromosome and autosomal RNA was measured as an indicator of relative transcription activity in larvae carrying various Sxl mutant alleles. Hyperactivity of X chromosomes was found in heteroallelic Sxlf#1/Sxlfhv#1 and homozygous Sxlf#2 female larvae. Sxlfhv#1 homozygotes, Sxlf#1/Sxl+ heterozygotes, heteroallelic Sxlf#2/Sxlf#2 as well as homozygous Sxlf#ba female larvae exhibited normal X chromosome transcription. Except for Sxlf#ba, there is a correlation between the viability of the mutants and the degree to which X-chromosome activity is elevated. Male larvae carrying the dominant male-specific lethal mutation SxlM#1 displayed X chromosomes only half as wide as those of control larvae. However, it could not be determined whether this property is the result of a lower transcription rate or of underreplication of the mutated X chromosomes. The results demonstrate that the Sxl gene plays an important role in controlling X-chromosome activity. The relationship among the various genes known to act in sex differentiation and dosage compensation is discussed.

Keywords

Developmental Biology Mutant Allele Transcription Activity Dosage Compensation Transcription Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ashburner, M.: Pattern of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments. Chromosoma (Berl.) 31, 356–376 (1970)CrossRefGoogle Scholar
  2. Baker, B.S., Ridge, K.A.: Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. Genetics 94, 383–423 (1980)PubMedGoogle Scholar
  3. Belote, J.M., Lucchesi, J.C.: Male-specific lethal mutations of Drosophila melanogaster. Genetics 94, 165–186 (1980a)Google Scholar
  4. Belote, J.M., Lucchesi, J.C.: Control of X chromosome transcription by the maleless gene in Drosophila. Nature (Lond.) 285, 573–575 (1980b)CrossRefGoogle Scholar
  5. Bridges, C.B.: The genetics of sex in Drosophila. In: Sex and internal secretions, pp. 55–93. Baltimore: Williams and Wilkins 1932Google Scholar
  6. Cline, T.W.: Two closely linked mutations in Drosophila melanogaster that are lethal to opposite sexes and interact with daughterless. Genetics 90, 683–698 (1978)PubMedGoogle Scholar
  7. Cline, T.W.: A product of the maternally-influenced Sex-lethal gene determines sex in Drosophila melanogaster. Genetics 91, s22 (1979a)Google Scholar
  8. Cline, T.W.: A male-specific lethal mutation in Drosophila melanogaster that transforms sex. Develop. Biol. 72, 266–275 (1979b)CrossRefPubMedGoogle Scholar
  9. Lindsley, D.L., Grell, E.H.: Genetic Variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. No. 627 (1968)Google Scholar
  10. Lucchesi, J.C.: Dosage compensation: transcription-level regulation of X-linked genes in Drosophila. Amer. Zool. 17, 685–693 (1977)Google Scholar
  11. Maroni, G., Plaut, W.: Dosage compensation in Drosophila melanogaster triploids. I. Autoradiographic study. Chromosoma (Berl.) 40, 361–377 (1973)Google Scholar
  12. Mukherjee, A.S., Beermann, W.: Synthesis of ribonucleic acid by the X-chromosomes of Drosophila melanogaster and the problem of dosage compensation. Nature (Lond.) 207, 785–786 (1965)Google Scholar
  13. Muller, H.J.: Evidence of the precision of genetic adaptation. Harvey Lecture Ser. 43, 165–229 (1950)Google Scholar
  14. Muller, H.J., Zimmering, S.: A sex-linked lethal without evident effect in Drosophila males but partially dominant in females. Genetics 45, 1001–1002 (1960)Google Scholar
  15. Skripsky, T., Lucchesi, J.C.: Females with sex-combs. Genetics 94, s98 (1980)Google Scholar
  16. Smith, P.D., Lucchesi, J.C.: The role of sexuality in dosage compensation in Drosophila. Genetics 61, 607–618 (1969)Google Scholar
  17. Snedecor, G.W., Cochran, W.G.: Statistical Methods. Ames: The Iowa State University Press 1967Google Scholar
  18. Stewart, B., Merriam, J.: Dosage compensation. In: The genetics and biology of Drosophila (M. Ashburner and T.R.F. Wright, eds.), Vol. 2d, pp. 107–140. London: Academic Press 1980Google Scholar
  19. Zimmering, S., Muller, H.J.: Studies on the action of the dominant Female-lethal Fl and of a seemingly less extreme allele Fls. Dros. Inf. Serv. 35, 103–104 (1961)Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • John C. Lucchesi
    • 1
  • Thomas Skripsky
    • 1
  1. 1.Department of Zoology and Genetics CurriculumThe University of North CarolinaChapel HillUSA

Personalised recommendations