Demonstration and solubilization of S-adenosylmethionine: γ-tocopherol methyltransferase from Capsicum chromoplasts
- 28 Downloads
Through the use of Capsicum chromoplast membranes, we report for the first time the direct methylation of γ-tocopherol into α-tocopherol in the presence of S-adenosylmethionine. Furthermore the S-adenosylmethionine: γ-tocopherol methyltransferase activity has been solubilized. On a protein basis, the activity recovered in the soluble preparation was higher than that bound to the membranes.
KeywordsProtein Basis Methyltransferase Activity Direct Methylation Soluble Preparation
- Camara B (1985) Meth Enzymol 111: 544 - 547Google Scholar
- Camara B, Bardat F, Monéger R (1982) Eur J Biochem 127: 255–258Google Scholar
- Camara B, Bardat F, Sèye A, d'Harlingue A, Monéger R (1982) Plant Physiol 70: 1562 - 1563Google Scholar
- Soll J, Douce R, Schultz G (1980) FEBS Lett 112: 243–246Google Scholar