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Plant Cell Reports

, Volume 4, Issue 1, pp 19–22 | Cite as

Heat shock proteins from cell cultures of higher plants and their somatic hybrids

  • S. V. Lopato
  • Yu.Yu. Gleba
Article
  • 31 Downloads

Abstract

The species specificity of heat shock proteins of callus cultures of Nicotiana chinensis, Nicotiana glauca, Nicotiana tabacum, Atropa belladonna, Lycopersicon peruvianum, as well as some somatic hybrids of A. belladonna + N. chinensis, was investigated by means of polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Despite the general similarity of electrophoretic mobility of heat shock proteins from different plants, a number of species-specific differences between the proteins of distantly related genera were found. Heat shock proteins may thus serve as genetic markers in somatic hybridization.

Keywords

Sodium Cell Culture Heat Shock Related Genus Polyacrylamide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

hs

heat shock

hsp

heat shock proteins

SDS

sodium dodecylsulfate

PAGel'phoresis

polyacrylamide gel electrophoresis

TCA

trichloroacetic aeid

NA-1(3;5;7;11;15)

Atropa belladonna + Nicotiana chinensis, clone 1(3;5;7;11;15)

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. V. Lopato
    • 1
  • Yu.Yu. Gleba
    • 1
  1. 1.Institute of BotanyAcademy of Sciences of the Ukrainian SSRKievUSSR

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