Advertisement

Human Genetics

, Volume 83, Issue 3, pp 297–299 | Cite as

Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10

  • D. M. Schonk
  • H. J. H. Kuijpers
  • E. van Drunen
  • C. H. van Dalen
  • A. H. M. Geurts van Kessel
  • R. Verheijen
  • F. C. S. Ramaekers
Short Communications

Summary

The antigen recognized by the monoclonal antibody Ki-67 is a proliferation-related nucleolus-associated constituent used as a marker for cycling cells in tumor diagnosis. Antibody Ki-67 reacts with human proliferating cells, but not with hamster and mouse cells. Expression of the Ki-67 antigen was studied in a panel of human-rodent somatic cell hybrids. The results indicate that a gene involved in the expression of the antigen is located on chromosome 10.

Keywords

Proliferate Cell Internal Medicine Monoclonal Antibody Metabolic Disease Somatic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barnard NJ, Hall PA, Lemoine NR, Kasar N (1987) Proliferative index in breast carcinoma determined in situ by Ki-67 immunostaining and its relationship to clinical and pathological variables. J Pathol 152:287–295Google Scholar
  2. Buys CHCM, Aanstoot GH, Nienhaus AJ (1984) The Giemsa-11 technique for species-specific chromosome differentiation: a simple stain modification leading to dependable direct and sequential staining procedures. Histochemistry 18:465–468Google Scholar
  3. Dongen JJM van, Versnel MA, Geurts van Kessel AHM, Smit EME, Hooykaas H, Hagemeijer A (1985) Leukemia: recent advances in biology and treatment. In: Gale RP, Golden DW (eds) USLA symposia on molecular and cellular biology, new series, vol 28. Liss, New York, pp 481–489Google Scholar
  4. Dunnen JT den, Jongbloed RJE, Geurts van Kessel AHM, Schoenmakers JGG (1985) Human lens γ-crystallin sequences are located in the p12→qter region of chromosome 2. Hum Genet 70:217–221Google Scholar
  5. Franklin WA, McDonald GB, Stein HO, Gatter KC, Jewell DP, Clarke LC, Mason DY (1985) Immunohistologic demonstration of abnormal colonic crypt cell kinetics in ulcerative colitis. Hum Pathol 16:1129–1132Google Scholar
  6. Fujimoto M, Fults DW, Thomas GA, Nakamura Y, Heilbrun MP, White R, Story JL, Naylor SL (1989) Loss of heterozygosity on chromosome 10 in human glioblastoma multiforme. Genomics 4:210–214Google Scholar
  7. Gatter KC, Dunnill MS, Gerdes J, Stein H, Mason DY (1986) New approach to assessing lung tumours in man. J Clin Pathol 39:590–593Google Scholar
  8. Gerdes J, Lemke H, Baish H, Wacker HH, Schwab U, Stein H (1984a) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710–1715Google Scholar
  9. Gerdes J, Dallenbach F, Lennert K (1984b) Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol 2:365–371Google Scholar
  10. Giangaspero F, Doglioni C, Rivano MT, Pileri S, Gerdes J, Stein H (1987) Growth fraction in human brain tumours defined by the monoclonal antibody Ki-67. Acta Neuropathol 74:179–182Google Scholar
  11. Mathew CGP, Chin KS, Easton DF, Thorpe K, Carter C, Liou GI, Fong SL, Bridges CDB, Haak H, Nieuwenhuyzen-Kruseman AC, Schifter D, Hansen HH, Telenius T, Telenius-Berg M, Ponder BAJ (1987) A linked genetic marker for multiple endocrine neoplasia type 2A on chromosome 10. Nature 328:527–528Google Scholar
  12. Ramaekers FCS, Puts JJG, Moesker O, Kant A, Huysmans A, Haag D, Jap PHK, Herman CJ, Vooijs GP (1983) Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview. Histochem J 15:691–713Google Scholar
  13. Simpson NE, Kidd KK, Goodfellow CE, McDermid H, Myers S, Kidd JR, Jackson CE, Duncan AMV, Farrer LA, Brasch K, Castiglione C, Genel M, Gertner J, Greenberg CR, Guselia JF Holden JJA, White BN (1987) Assigment of multiple endocrine neoplasia type 2A to chromosome 10 by linkage. Nature 328:528–530Google Scholar
  14. Spurr NK, Goodfellow PN, Nurse P, Lee M (1987) Assignment of the human homoloque of the yeast cell cycle control gene CDC2 to chromosome 10 (abstract). (9th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 46:698Google Scholar
  15. Verheijen R, Kuijpers HJH, Schlingemann RO, Boehmer ALM, Driel R van, Brakenhoff GJ, Ramaekers FCS (1989) Ki-67 detects a nuclear matrix-associated proliferation-related antigen. I. Intracellular localization during interphase. J Cell Sci 92:123–130Google Scholar
  16. Wieacker P, Davies KE, Cooke HJ, Pearson PL, Williamson R, Bhattacharya S, Zimmer J, Ropers HH (1984) Toward a complete linkage map of the human X chromosome; regional assignment of 16 cloned single-copy DNA sequences employing a panel of somatic cell hybrids. Am J Hum Genet 36:265–276Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • D. M. Schonk
    • 1
  • H. J. H. Kuijpers
    • 2
  • E. van Drunen
    • 3
  • C. H. van Dalen
    • 1
  • A. H. M. Geurts van Kessel
    • 3
  • R. Verheijen
    • 2
  • F. C. S. Ramaekers
    • 2
  1. 1.Department of Human GeneticsUniversity Hospital of NijmegenNijmegenThe Netherlands
  2. 2.Department of PathologyUniversity Hospital of NijmegenNijmegenThe Netherlands
  3. 3.Department of Cell Biology and GeneticsErasmus UniversityRotterdamThe Netherlands

Personalised recommendations