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Human Genetics

, Volume 76, Issue 2, pp 157–164 | Cite as

Application of cloned satellite DNA sequences to molecular-cytogenetic analysis of constitutive heterochromatin heteromorphisms in man

  • Yuri B. Yurov
  • S. P. Mitkevich
  • I. A. Alexandrov
Original Investigations

Summary

The cloned alpha-satellite DNA sequences were used to evaluate the specificity and possible variability of repetitive DNA in constitutive heterochromatin of human chromosomes. Five probes with high specificity to individual chromosomes (chromosomes 3, 11, 17, 18, and X) were in situ hybridized to metaphase chromosomes of different individuals. The stable position of alpha-satellite DNA sequences in heterochromatic regions of particular chromosomes was found. Therefore, the chromosome-specific alpha-satellite DNA sequences may be used as molecular markers for heterochromatic regions of certain human chromosomes. The homologous chromosomes of many individuals were characterized by cytologically visible heteromorphisms of hybridization intensity with chromosome-specific alpha-satellite DNA sequences. A special analysis of hybridization between homologues with morphological differences provided the evidence for a high resolution power of the in situ hybridization technique for evaluation of chromosome heteromorphisms. The approaches for detection of heteromorphisms in cases without morphological differences between homologues are discussed. The results obtained indicate that constitutive heterochromatin of human chromosomes has a variable amount of alphasatellite DNA sequences. In situ hybridization of cloned satellite DNA sequences may be used as a new general approach to analysis of chromosome heteromorphisms in man.

Keywords

Molecular Marker High Specificity Human Chromosome Morphological Difference Variable Amount 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alexandrov IA, Yurov YB, Mitkevich SP, Gindilis VM (1986a) Chromosomal organization of human alphoid DNA. Dokl Acad Nauk SSSR 288:242–245Google Scholar
  2. Alexandrov IA, Yurov YB, Mitkevich SP, Gindilis VM (1986b) Cloned fragment of human alphoid DNA — a molecular marker of pericentromeric region of chromosome 18. Genetika (Moskva) 5:868–876Google Scholar
  3. Bostock CJ, Gosden JR, Mitchell AR (1978) Localization of a male-specific DNA fragment to a sub-region of the human Y chromosome. Nature 27:324–328Google Scholar
  4. Chandler ME, Yunis JJ (1978) A high resolution in situ hybridization technique for direct visualization of labelled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet 22: 352–356Google Scholar
  5. Cooke HJ, Hindley J (1979) Cloning of human satellite III DNA: different components are on different chromosomes. Nucleic Acids Res 10:3177–3197Google Scholar
  6. Corneo G, Ginelli E, Zardi L (1973) Satellite and repeated sequences in human DNA. In: Pfeiffer RA (ed) Modern aspects of cytogenetics: constitutive heterochromatin in man. Schattauer, Stuttgart New York, pp 29–37Google Scholar
  7. Erdtmann B (1982) Aspects of evaluation, significance, and evolution of human C-band heteromorphism. Hum Genet 61:281–294Google Scholar
  8. Erickson R, Wolfe J, Darling S, Craig I, Rigby D, Goodfellow D (1984) Cloning centromeric sequences from the human Y chromosome. Am J Hum Genet 36:136Google Scholar
  9. Gosden JR, Lawrie SS, Cooke HJ (1981) A cloned DNA sequences in human chromosome heteromorphisms. Cytogenet Cell Genet 29:32–39Google Scholar
  10. Human Gene Mapping 8 (1985) (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:No 1-4Google Scholar
  11. Jacobs PA (1977) Human chromosome heteromorphisms. Prog Med Genet 2:251–274Google Scholar
  12. Lau YF, Huang JC, Dozy MA, Kan YW (1984) A rapid screening test for antenatal sex determination. Lancet I:14–16Google Scholar
  13. Manuelidis L (1978) Chromosomal localization of complex and simple repeated human DNAs. Chromosoma 66:23–32Google Scholar
  14. Singer M (1982) Highly repeated sequences in mammalian genomes. Int Rev Cytol 76:67–112Google Scholar
  15. Vorsanova SG, Yurov YB, Alexandrov IA, Mitkevich SP, Tirskaia AF (1986) 18p- syndrome: an unusual case and diagnosis by in situ hybridization with chromosome 18-specific alphoid DNA sequences. Hum Genet 72:185–187Google Scholar
  16. Willard HF (1985) Chromosome-specific organization of human alpha-satellite DNA. Am J Hum Genet 37:524–532Google Scholar
  17. Yakovlev AG (1983) Cloned repeated DNA sequences and experimental search for molecular genetic markers of human chromosomes. Thesis for doctor degree, Moscow, 1983Google Scholar
  18. Yang TP, Hansen SK, Oishi KK, Ryder OA, Hamkalo BA (1982) Characterization of a cloned repetitive DNA sequences concentrated on the human X chromosome. Proc Natl Acad Sci USA 79: 6593–6597Google Scholar
  19. Yurov YB (1984) In situ hybridization of cloned repetitive DNA sequences and differential staining of human chromosomes. Biull Eksp Biol Med 97:595–598Google Scholar
  20. Yurov YB, Yakovlev AG, Zaitzev IZ, Mitkevich SP, Alexandrov IA, Shapiro YuA, Kramerova IA, Gindilis VM (1986a) The cloned fragment of reiterated human DNA specific to centromeric heterochromatin of chromosome 3. Mol Genet Microbiol Virusol (USSR) 7:19–23Google Scholar
  21. Yurov YB, Alexandrov IA, Mitkevich SP, Kroumina AR (1986b) The cloned sequences of satellite III DNA highly specific to centromeric heterochromatin of human chromosome 9. Mol Genet Microbiol Virusol (USSR) 8:9–11Google Scholar
  22. Zaitzev IZ (1984) Structural analysis of cloned molecular markers of human chromosomes. Thesis for doctor degree, Moscow, 1984Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Yuri B. Yurov
    • 1
  • S. P. Mitkevich
    • 1
  • I. A. Alexandrov
    • 1
  1. 1.All-Union Research Center of Mental HealthAcademy of Medical Sciences of USSRMoscowUSSR

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