European Biophysics Journal

, Volume 17, Issue 4, pp 191–199 | Cite as

Measurement of an intracellular pH rise after fertilization in crab eggs using 31P-NMR

  • M. Hervé
  • M. Goudeau
  • J. M. Neumann
  • J. C. Debouzy
  • H. Goudeau
Article
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Accepted:

Abstract

The effect of fertilization upon the intracellular pH, pHi, in crab ovulated eggs was examined by 31P-NMR. The pHi values were obtained from the chemical shift differences between the phosphoarginine PA resonance and the inorganic phosphate Pi resonance. The detection of the Pi peak was accomplished by Hahn spin-echo experiments in order to cancel the broad signal arising from phosphoproteins which overlaps the Pi signal. The average pHi of the unfertilized unactivated eggs was 6.55 and a rise of 0.12 pH unit occurred after fertilization.

Key words

Crab eggs fertilization 31P-NMR intracellular pH 31P transverse relaxation in cells 
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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Hervé
    • 1
    • 2
  • M. Goudeau
    • 1
    • 3
    • 4
  • J. M. Neumann
    • 1
  • J. C. Debouzy
    • 1
  • H. Goudeau
    • 1
    • 3
    • 4
  1. 1.Service de BiophysiqueCEN SaclayGif-sur-Yvette CédexFrance
  2. 2.UFR Santé Médecine Biologie HumaineUniversité Paris XIIIBobignyFrance
  3. 3.UA-CNRS 686RoscoffFrance
  4. 4.Station Marine de RoscoffRoscoffFrance

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