Human Genetics

, Volume 71, Issue 3, pp 254–258 | Cite as

The isolation, characterisation, and chromosomal assignment of the gene for human 3-hydroxy-3-methylglutaryl coenzyme A reductase, (HMG-CoA reductase)

  • S. E. Humphries
  • F. Tata
  • I. Henry
  • F. Barichard
  • M. Holm
  • C. Junien
  • R. Williamson
Original Investigations

Summary

We have used a cDNA clone for Chinese hamster 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase to isolate a genomic recombinant for human HMG-CoA reductase. The identity of the gene was confirmed by partial sequence analysis. Several unique fragments that will be useful for restriction fragment length polymorphism (RFLP) studies were identified. In situ hybridisation of a 2.6kb unique fragment of the gene to human metaphase chromosomes localised the human HMGCoA reductase gene to human chromosome 5q12.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. E. Humphries
    • 1
  • F. Tata
    • 1
  • I. Henry
    • 2
  • F. Barichard
    • 2
  • M. Holm
    • 1
  • C. Junien
    • 2
  • R. Williamson
    • 1
  1. 1.Department of BiochemistrySt. Mary's Hospital Medical SchoolLondonEngland
  2. 2.Groupe de Recherches de Biologie Prenatale, INSERM U 73Chateau de LongchampParisFrance

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