The distribution of tritium-labeled aflatoxin B1, dihydro-aflatoxin B1, and tetrahydrodesoxoaflatoxin B1 in liver cells cultured in vitro from the chicken embryo

  • Kiyoshi Terao
  • Mikio Yamazaki
  • Komei Miyaki


The distribution patterns of 3H-aflatoxin B1, B2 and tetrahydrodesoxoaflatoxin B1 (4H B1) in the cultured liver cells originated from the 12-day-old chicken embryo were demonstrated. The fact that the hepatocytes were more heavily labeled than the mesenchymal cells by the incorporation of 3H-aflatoxins correlate well with the observation that the hepatocytes were more susceptible than the mesenchymal cells to the aflatoxins. There were no significant differences in the pattern of incorporation of 3H-aflatoxin B1, B2 and 4H B1 into the hepatocyte, while the three mycotoxins show a wide variation in their inhibitory action in RNA synthesis. It may be concluded from the above mentioned facts that the toxicity of aflatoxins to the liver cells in vitro is closely related to the membrane permeability and the active portion of the molecule of aflatoxins, such as the double bond in the difuran structure and the carbonyl group in the cyclopentenone portion of B1.


Public Health Cancer Research Carbonyl Double Bond Liver Cell 
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Die Verteilung von Tritium-markiertem Aflatoxin B1, Dihydro-Aflatoxin B1 und Tetrahydrodedesoxoaflatoxin B1 in Lebenzellen von Hühnerembryonen in vitro


Die Tatsache, daß die Leberzellen stärker durch Inkorporation von 3H-Aflatoxin markiert werden als die Mesenchymzellen entspricht der Beobachtung, daß Leberzellen gegenüber Aflatoxinen anfälliger sind als die Mesenchymzellen. Es zeigten sich keine signifikanten Unterschiede hinsichtlich der Inkorporation von 3H-Aflatoxin B1, B2 und 4HB1 in die Leberzellen, während diese drei Mycotoxine eine weite Variation hinsichtlich ihrer Hemmwirkung auf die RNS-Synthese aufwiesen. Man kann also daraus den Schluß ziehen, daß die Toxizität von Aflatoxinen für Leberzellen in vitro eng zusammenhängt mit der Membrandurchlässigkeit und dem aktiven Teil des Aflatoxinmoleküls bzw. der Dop pelbindung in Difuranstruktur und der Carbonylgruppe in dem Cyclopentenon-Anteil von B1.


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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Kiyoshi Terao
    • 1
  • Mikio Yamazaki
    • 2
  • Komei Miyaki
    • 2
  1. 1.Department of Pathology, School of MedicineChiba UniversityChibaJapan
  2. 2.Institute of Food MicrobiologyChiba UniversityChibaJapan

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