Human Genetics

, Volume 76, Issue 3, pp 236–239 | Cite as

Human δ-aminolevulinate dehydratase: chromosomal localization to 9q34 by in situ hybridization

  • Venkateswara R. Potluri
  • Kenneth H. Astrin
  • James G. Wetmur
  • David F. Bishop
  • Robert J. Desnick
Original Investigations


The structural gene for human δ-aminolevulinate dehydratase (ALA-D) has been localized to chromosomal region 9q34 by in situ hybridization using a [125I]-labeled human δ-aminolevulinate dehydratase cDNA. Of the 150 silver grains analyzed, 25% were localized to chromosome 9q, while 12% and 8% were on chromosomes 1p and 13q, respectively. The single chromosomal region q34 had over 90% of the total grains observed on chromosome 9. In contrast, the grains on chromosomes 1p and 13q were dispersed, consistent with the absence of any human ALD-D pseudogenes. Southern blot analysis of somatic cell hybrids informative for ALA-D (Wang et al. 1985) also was consistent and supported the finding of only one locus for this heme biosynthetic enzyme.


Internal Medicine Blot Analysis Metabolic Disease Somatic Cell Structural Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Venkateswara R. Potluri
    • 1
  • Kenneth H. Astrin
    • 1
  • James G. Wetmur
    • 2
  • David F. Bishop
    • 1
  • Robert J. Desnick
    • 1
  1. 1.Division of Medical Genetics, Jack and Lucy Department of PediatricsMount Sinai School of MedicineNew YorkUSA
  2. 2.Department of MicrobiologyMount Sinai School of MedicineNew YorkUSA

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