Molecular and General Genetics MGG

, Volume 242, Issue 6, pp 681–688 | Cite as

Map-based cloning in crop plants. Tomato as a model system: I. Genetic and physical mapping of jointless

  • Rod A. Wing
  • Hong-Bin Zhang
  • Steven D. Tanksley
Original Articles

Abstract

A map-based cloning scheme is being used to isolate the jointless (j) gene of tomato. The jointless locus is defined by a single recessive mutation that completely suppresses the formation of the fruit and flower pedicel and peduncle abscission zone. jointless was mapped in an F2 population of an interspecific cross between Lycopersicon esculentum and Lycopersicon pennellii to a 7.1 cM interval between two restriction fragment length polymorphism (RFLP) markers TG523 and TG194. Isogenic DNA pools were then constructed from a subset of the mapping population and screened with 800 random decamers for random amplification of polymorphic DNA (RAPD) polymorphisms. Five new RAPD markers were isolated and mapped to chromosome 11, two of which were mapped within the targeted interval. One marker, RPD158, was mapped 1.5 cM to the opposite side of jointless relative to TG523 and thus narrowed the interval between the closest flanking markers to 3.0 cM. Physical mapping by pulse-field gel electrophoresis using TG523 and RPD158 as probes demonstrated that both markers hybridize to a common 600 kb SmaI restriction fragment. This provided an estimate of 200 kb/cM for the relationship between physical and genetic distances in the region of chromosome 11 containing the j locus. The combined results provide evidence for the feasibility of the next step toward isolation of the jointless gene by map-based cloning — a chromosome walk or jump to jointless.

Key words

Lycopersicon esculentum Map-based cloning RFLP/RAPD mapping Physical mapping Abscission 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Addicott FT (1982) Abscission. University of California Press, BerkeleyGoogle Scholar
  2. Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278Google Scholar
  3. Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HN, Somerville CR (1992) Map-based cloning of gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258:1353–1355Google Scholar
  4. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218Google Scholar
  5. Bernatzky R, Tanksley SD (1986) Methods for detection of single or low copy sequence in tomato on Southern blots.Plant Mol Biol Rep 4:37–41Google Scholar
  6. Butler L (1936) Inherited characters in the tomato. J Hered 27:25–26Google Scholar
  7. Charlieu J-P, Laurent A-M, Orti R, Viegas-Pequignot E, Bellit M, Roizes G (1993) A 37-kb fragment common to the pericentromeric region of human chromosomes 13 and 21 and to the ancestral inactive centromere of chromosome 2. Genomics 15:576–581Google Scholar
  8. Chu G, Vollrath D, Davis RW (1986) Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234:1582–1585Google Scholar
  9. Feinberg AP, Vogelstein S (1984) A technique for radio-labeling DNA fragments to high specific activity. Anal Biochem 137:266–267Google Scholar
  10. Ganal MW, Young ND, Tanksley SD (1989) Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400Google Scholar
  11. Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res 19:6553–6558Google Scholar
  12. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4:1251–1261Google Scholar
  13. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  14. Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340Google Scholar
  15. Martin GB, Ganal MW, Tanksley SD (1992) Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci. Mol Gen Genet 223:25–32Google Scholar
  16. McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Rep 5:81–84Google Scholar
  17. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88:9828–9832Google Scholar
  18. Paterson AH, Wing RA (1993) Genome mapping in plants. Curr Opin Biotechnol 4:142–147Google Scholar
  19. Reed KC, Mann DA (1985) Rapid transfer from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221Google Scholar
  20. Rick CM (1956) Genetic and systematic studies on accessions of Lycopersicon from the Galapagos Islands. Am J Bot 43:687–696Google Scholar
  21. Rick CM (1971) Some cytogenetic features of the genome in diploid plant species. Stadler Genet Symp 1:153–174Google Scholar
  22. Rick CM, Yoder JI (1988) Classical and molecular genetics of tomato: highlights and perspectives. Annu Rev Genet 22:281–300Google Scholar
  23. Roberts JA, Schindler CB, Tucker GA (1984) Ethylene-promoted tomato flower abscission and the possible involvement of an inhibitor. Planta 160:159–163Google Scholar
  24. Rommens JM, Iannuzzi MC, Kerem B, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hidaka N, Zsiga M, Buchwald M, Riordan JR, Tsui LC, Collins FS (1989) Identification of the cystic fibrosis gene: chromosome walking and jumping. Science 245:1059–1065Google Scholar
  25. Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene 12. Theor Appl Genet 78:755–759Google Scholar
  26. Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I 2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185Google Scholar
  27. Sexton R, Roberts JA (1982) Cell biology of abscission. Annu Rev Plant Physiol 33:133Google Scholar
  28. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517Google Scholar
  29. Tanksley SD, Ganal MW, Prince J, de Vicente M, Bonierbale MW, Broun P, Fulton T, Giovannoni J, Grandillo S, Martin G, Messeguer R, Miller J, Miller L, Paterson A, Pineda O, Roder M, Wing R, Wu W, Young N (1992) High density molecular linkage maps of the tomato and potato genomes: biological inferences and practical applications. Genetics 132:1141–1160Google Scholar
  30. Trask B, Fertitta A, Christensen M, Youngblom J, Bergmann A, Copeland A, de Jong R, Mohrenweisen H, Olsen A, Carrano A, Tynan K (1993) Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 gene or DNA markers. Genomics 15:133–145Google Scholar
  31. Wicking C, Williamson B (1991) From linked marker to gene. Trends Genet 7:288–293Google Scholar
  32. Wing RA, Rastogi VK, Zhang HB, Paterson AH, Tanksley SD (1993) An improved method of plant megabase DNA isolation in agarose microbeads suitable for physical mapping and YAC cloning. Plant J, 4:893–898Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Rod A. Wing
    • 1
    • 2
  • Hong-Bin Zhang
    • 1
  • Steven D. Tanksley
    • 2
  1. 1.Soil & Crop Sciences DepartmentTexas A&M UniversityCollege StationUSA
  2. 2.Plant Science Center & Department of Plant Breeding and BiometryCornell UniversityIthacaUSA

Personalised recommendations