Molecular and General Genetics MGG

, Volume 242, Issue 6, pp 631–640 | Cite as

Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae

  • Satoshi Yoshida
  • Yoshikazu Ohya
  • Akihiko Nakano
  • Yasuhiro Anraku
Original Articles

Abstract

Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

Key words

Staurosporine Phosphatidylinositol 4-kinase Protein kinase C Cell lysis Cell cycle 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Satoshi Yoshida
    • 1
  • Yoshikazu Ohya
    • 1
    • 2
  • Akihiko Nakano
    • 1
  • Yasuhiro Anraku
    • 1
  1. 1.Department of Biology, Faculty of ScienceUniversity of TokyoTokyoJapan
  2. 2.Department of GeneticsStanford University School of MedicineStanfordUSA

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