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Molecular and General Genetics MGG

, Volume 237, Issue 1–2, pp 261–272 | Cite as

Characterization of the promoter from the single-copy gene encoding ferredoxin-NADP+-oxidoreductase from spinach

  • R. Oelmüller
  • C. Bolle
  • A. K. Tyagi
  • N. Niekrawietz
  • S. Breit
  • R. G. Herrmann
Article

Summary

We describe a genomic DNA segment from spinach that bears part of the single-copy gene for ferredoxin-NADP+-oxidoreductase (FNR) including a 3.4 kb promoter sequence. Dissection of this DNA segment and its analysis in GUS (β-glucuronidase) gene fusions in transgenic tobacco demonstrated that the promoter differs in structure from all other promoters for thylakoid protein genes studied to date. Two regions with light-responsive elements were identified. One is located within the first 118 by upstream of the transcription initiation site. A second fragment covering nucleotide positions − 220 to − 119 is capable of conferring light-dependent GUS gene expression on two different minimal promoters. The latter fragment binds a trans-acting factor in gel-shift assays. None of the fragments carries cis elements known from other genes to be involved in light-controlled expression. Comparison of the light responsiveness of GUS gene fusions controlled by the −753/+231 and −118/+231 regions indicates that they respond differentially to phytochrome-dependent signals and that their expression in tobacco is not restricted to tissue with functional chloroplasts.

Key words

Cis regulatory elements Ferredoxin-NADP+-oxidoreductase Light-responsive elements Pfr-induced signal transduction Promoter analysis 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • R. Oelmüller
    • 1
  • C. Bolle
    • 1
  • A. K. Tyagi
    • 1
  • N. Niekrawietz
    • 1
  • S. Breit
    • 1
  • R. G. Herrmann
    • 1
  1. 1.Botanisches Institut der Ludwig-Maximilians-UniversitätMünchen 19FRG

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