Human Genetics

, Volume 74, Issue 3, pp 239–243 | Cite as

DNA finger printing by oligonucleotide probes specific for simple repeats

  • S. Ali
  • C. R. Müller
  • J. T. Epplen
Original Investigations


Interspersed simple repetitive DNA is a convenient genetic marker for analysis of restriction fragment length polymorphisms (RFLPs) because of the numbers and the frequencies of its alleles. Oligonucleotide probes specific for variations of the GA C T A simple repeats have been designed and hybridized to a panel of human DNAs digested with various restriction enzymes. Numerous RFLPs were demonstrated in AluI and MboI digested DNA with “pure” GATA oligonucleotides as probes. The optimal length of the probe for RFLP analysis was 20 bases taking into account fragment lengths (1.5-7 kilobases = kb), signal to background ratio, and number of clearly evaluable RFLPs. By using different restriction enzymes individual-specific hybridization patterns (“DNA fingerprints”) can be established. Hypervariable simple repeat fragments are stably inherited in a Mendelian fashion. Advantages of this method are discussed.


Restriction Enzyme Fragment Length Length Polymorphism Restriction Fragment Length Polymorphism Restriction Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • S. Ali
    • 1
  • C. R. Müller
    • 2
  • J. T. Epplen
    • 1
  1. 1.Junior Research UnitMax-Planck-Institut für ImmunbiologieFreiburgFederal Republic of Germany
  2. 2.Institut für Humangenetik der UniversitätWürzburgFederal Republic of Germany

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