, Volume 26, Issue 2, pp 155–158 | Cite as

Altered biochemical responses by rat Sertoli cells and peritubular cells cultured under simulated diabetic conditions

  • J. C. Hutson


The purpose of the present study was to determine whether the secretion of androgen-binding protein and lactate by 16-day-old rat Sertoli cells is altered when these cells are cultured in simulated diabetic conditions. Incorporation of 3H-uridine into RNA by peritubular cells cultured in simulated diabetic conditions was also studied. The cells were exposed to various concentrations of glucose, β-hydroxybutyrate, sodium bicarbonate (to alter the pH of the medium), mannitol (to influence the osmolarity of the medium), or a combination of these compounds. All of the metabolic parameters when tested alone or in combination were capable of increasing lactate secretion by Sertoli cells above control values. Basal secretion of androgen-binding protein, however, was not influenced by any individual component or when all components were tested together. FSH-stimulated levels of androgen-binding protein secretion was depressed only when the Sertoli cells were exposed to all the components simultaneously. Incorporation of uridine by peritubular cells was decreased by exposing the cells to butyrate or mannitol, while no effect was observed with glucose treatment. These results indicate that Sertoli cell and peritubular cell function can be directly altered by several specific metabolic parameters associated with diabetes.

Key words

Sertoli cells diabetes androgen-binding protein 


  1. 1.
    Kolodny RC (1974) Sexual dysfunction in diabetic men. Diabetes 23: 306–309Google Scholar
  2. 2.
    Rubin A, Babbott D (1958) Impotence and diabetes mellitus. JAMA 168: 498–500Google Scholar
  3. 3.
    Rosenmann E, Palti Z, Teitelbaum A, Cohen AM (1974) Testicular degeneration in genetically selected sucrose-fed diabetic rats. Metabolism 23: 343–348Google Scholar
  4. 4.
    Fritz IB, Rommerts FG, Louis BG, Dorrington JH (1976) Regulation by FSH and dibutyryl c-AMP of the formation of androgenbinding protein in Sertoli cell-enriched cultures. J Reprod Fertil 46: 17–24Google Scholar
  5. 5.
    Hagenas L, Ritzen EM, Ploen L, Hansson V, Neyfeh SN (1975) Sertoli cell origin of androgen-binding protein. Mol Cell Endocrinol 2: 339–350Google Scholar
  6. 6.
    Steinberger A, Heindel JJ, Lindsey JN, Elkington JSH, Sanborn BM, Steinberger E (1975) Isolation and culture of FSH-responsive Sertoli cells. Endoc Res Commun 2: 261–272Google Scholar
  7. 7.
    Fakunding JL, Tindall DJ, Dedman JR, Mena CR, Means AR (1976) Biochemical Actions of FSH in the Sertoli cell of the rat testis. Endocrinology 98: 392–402Google Scholar
  8. 8.
    Lacroix M, Smith FE, Fritz IB (1977) Secretion of plasminogen activator by Sertoli cell enriched cultures. Mol Cell Endocrinol 9: 227–236Google Scholar
  9. 9.
    Hutson JC, Stocco DM (1981) Regulation of Sertoli cell function by thyrotropin. Biol Reprod 25: 303–306Google Scholar
  10. 10.
    Skinner MK, Griswold MD (1980) Sertoli cells synthesize and secrete transferrin-like protein. J Biol Chem 255: 9523–9525Google Scholar
  11. 11.
    Dorrington JH, Fritz IB, Armstrong DT (1976) Testicular estrogens: Synthesis by isolated Sertoli cells and regulation by FSH. In: Spilman CH, Lobl I, Kinton KI (eds), Regulatory mechanisms of male reproductive physiology, Exerpta Medica, Amsterdam, pp 63–70Google Scholar
  12. 12.
    Tcholakian RK, Steinberger A (1979) In vitro metabolism of testosterone by cultured Sertoli cells and the effect of FSH. Steroids 33: 495–526Google Scholar
  13. 13.
    Grootegoed JA, Jutte NHPM, Jansen R, Heusdens FA, Rommerts FFG, van der Molen HJ (1981) Biochemistry of Spermatogenesis. The supporting role of Sertoli cells. In: van der Molen HJ, Klopper A, Lunenfeld B (eds) Hormonal factors in fertility, infertility and contraception. Elsevier, Amsterdam, pp 169–183Google Scholar
  14. 14.
    Mita M, Price M, Hall PF (1982) Stimulation by FSH of synthesis of lactate by Sertoli cells from rat testis. Endocrinology 110: 1535–1541Google Scholar
  15. 15.
    Kark JF, Griswold MD (1980) Actions of insulin and vitamin A on Sertoli cells. Biochem J 186: 1001–1003Google Scholar
  16. 16.
    Means AR, Fakunding JL, Huckins C, Tindall DJ, Vitale R (1976) FSH, the Sertoli cells, and spermatogenesis. Rec Prog Hor Res 32: 477–528Google Scholar
  17. 17.
    Elkington JSH, Sanborn BM, Martin MW, Chowdhury AK, Steinberger E (1977) Effect of testosterone propionate on ABP levels in rats hypophysectomized at different ages using individual sampling. Mol Cell Endocrinol 6: 203–209Google Scholar
  18. 18.
    Weddington SC, Hansson V, Ritzen EM, Hagenas L, French FS, Neyfeh SN (1975) Sertoli cell secretory function after hypophysectomy. Nature 254: 145–146Google Scholar
  19. 19.
    Jutte NHPM, Jansen R, Grootegoed JA, Rommerts FFG, Clausen OPF, van der Molen HJ (1982) Regulation of survival of rat pachytene spermatocytes by lactate supply from Sertoli cells. J Reprod Fertil 65: 431–438Google Scholar
  20. 20.
    Hutson JC, Stocco DM, Campbell GT, Wagoner J (1983) Sertoli cell function in diabetic, insulin treated diabetic and semi-starved rats. Diabetes 32: 112–116Google Scholar
  21. 21.
    Murray FT, Orth J, Gunsalus G, Musto NA, Weisz J, Li J, Jefferson LS, Bardin CW (1981) The pituitary-testicular axis in the streptozotocin diabetic male rat: evidence for gonadotroph, Sertoli cell and Leydig cell dysfunction. Int J Androl 4: 265–280Google Scholar
  22. 22.
    Hutson JC, Stocco DM (1981) Peritubular cell influence on the efficiency of ABP secretion by Sertoli cells in culture. Endocrinology 108: 1362–1368Google Scholar
  23. 23.
    Dorrington JH, Roller NF, Fritz IB (1975) Effects of FSH on cultures of Sertoli cell preparations. Mol Cell Endocrinol 3: 57–70Google Scholar
  24. 24.
    Ritzen EM, French FS, Weddington SC, Neyfeh SN, Hansson V (1974) Steroid binding in polyacrylamide gels: quantitation at steady state conditions. J Biol Chem 249: 6579–6604Google Scholar
  25. 25.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  26. 26.
    Brunk CF, Jones KC, James TW (1979) Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 92: 497–500Google Scholar
  27. 27.
    Phieffer JC, Alger EA, Boccabella Av (1981) Origin of collagen in the peritubular boundary tissue of the rat testis. Anat Rec 199: 200 AGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. C. Hutson
    • 1
  1. 1.Department of AnatomyTexas Tech University Health Sciences CenterLubbockUSA

Personalised recommendations