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Molecular and General Genetics MGG

, Volume 242, Issue 3, pp 241–249 | Cite as

The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal -map and is a new member of the AMP-binding protein family

  • Martin Fulda
  • Ernst Heinz
  • Frank P. Wolter
Original Articles

Abstract

The fadD gene of Escherichia coli K12 was cloned and sequenced. The gene was identified by its ability to complement the corresponding mutant and by measuring the enzymatic activity after its expression in this mutant. The deduced polypeptide sequence exhibits similarity to other long chain acyl-CoA (coenzyme A) synthetases and a variety of other proteins, which together form a family of AMP-binding proteins. This family is extended by several new members and subdivided into four groups. fadD is assigned to a subgroup that does not include long chain acyl-CoA synthetases from eukaryotic organisms.

Key words

FadD acyl-CoA synthetase AMP-binding Escherichia coli K12 Sequence 

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References

  1. Abe T, Fujino T, Fukuyama R, Minoshima S, Shimizu N, Toh H, Suzuki H, Yamamoto T (1992) Human long-chain acyl-CoA synthetase: structure and chromosomal location. J Biochem 111:123–128Google Scholar
  2. Babbitt PC, Kenyon G, Martin B, Charest H, Sylvestre M, Scholten JD, Chang K-H, Liang P-H, Dunaway-Marino D (1992) Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl: adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases. Biochemistry 31:5594–5604Google Scholar
  3. Bachmann BJ (1990) Linkage map of Escherichia coli K-12 edition 8. Microbiol Rev 54:130–197Google Scholar
  4. Becker-André M, Schulze-Lefert P, Hahlbrock K (1991) Structural comparison, modes of expression, and putative cis-acting elements of the two 4-coumarate:CoA ligase genes in potato. J Biol Chem 266:8551–8559Google Scholar
  5. Black PN (1992) Physical map location of the Escherichia coli gene encoding acyl coenzyme A synthetase. J Bacteriol 174:7875Google Scholar
  6. Black PN, DiRusso CC, Metzger AK, Heimert TL (1992) Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase. J Biol Chem 267:25513–25520Google Scholar
  7. Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heynecker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. Gene 2:95–113Google Scholar
  8. Clark SP (1992) MALIGNED: a multiple sequence alignment editor. Cabios 8:535–538Google Scholar
  9. Connerton IF, Fincham JRS, Sandeman RA, Hynes MJ (1990) Comparison and cross-species expression of the acetyl-CoA synthetase genes of the ascomycete fungi Aspergillus nidulans and Neurospora crassa. Mol Microbiol 4:451–460Google Scholar
  10. Coque JJR, Martin JF, Calzada JG, Liras P (1991) The cephamycin biosynthetic genes pcbAB encoding a large multidomain peptide synthetase and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum. Mol Microbiol 5:1125–1133Google Scholar
  11. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs of the VAX. Nucleic Acids Res 12:387–395Google Scholar
  12. De Virgilio C, Buerckert N, Barth G, Neuhaus JM, Boller T, Wiemken A (1992) Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast 8:1043–1051Google Scholar
  13. De Wet JR, Wood KV, De Luca M, Helsinki DR, Subrami S (1987) Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 7:725–737Google Scholar
  14. Diéz B, Gutiérrez S, Barredo JL, van Solingen P, van der Voort LHN, Martin JF (1990) The cluster of penicillin biosynthesis genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pchC and penDE genes. J Biol Chem 265:16358–16365Google Scholar
  15. Di Russo CC, Heimert TL, Metzger AK (1992) Characterization of FadR, a global transcriptional regulator of fatty acid metabolism in Escherichia coli. J Biol Chem 267:8685–8691Google Scholar
  16. Doolittle RF (1986) Of URFs and ORFs — a primer on how to analyze derived amino acid sequences. University Science Books, Mill Valley, Calif.Google Scholar
  17. Driscoll JR, Taber HW (1992) Sequence organization and regulation of the Bacillus subtilis menBE operon. J Bacteriol 174:5063–5071Google Scholar
  18. Duronio RJ, Knoll LJ, Gordon J (1992) Isolation of a Saccharomyces cerevisiae long chain fatty acyl:CoA synthetase gene (FAA1) and assessment of its role in protein N-myristoylation. J Cell Biol 117:515–529Google Scholar
  19. Eggen RIL, Geerling ACM, Boshoven ABP, De Vos WM (1991) Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli. J Bacteriol 173:6383–6389Google Scholar
  20. Farrell DH, Mikesell P, Actis LA, Crosa JH (1990) A regulatory gene angR of the iron uptake system of Vibrio anguillarum: similarity with phage P22 cro and regulation by iron. Gene 86:45–51Google Scholar
  21. Favaloro JM, Marshall VM, Crewther PE, Coppel RL, Kemp DJ, Anders RF (1989) cDNA sequence predicting an octapeptiderepeat antigen of Plasmodium falciparum. Mol Biochem Parasitol 32:297–299Google Scholar
  22. Fujino T, Yamamoto T (1992) Cloning and functional expression of a novel long-chain acyl-CoA synthetase expressed in brain. J Biochem 111:197–203Google Scholar
  23. Gold L, Stormo G (1987) Translational initiation. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium — cellular and molecular biology, American Society of Microbiology, Washington DC, pp 1302–1307Google Scholar
  24. Gutiérrez S, Diéz B, Montenegro E, Martin JF (1991) Characterization of the Cephalosporium acremonium pcbAB gene encoding alpha-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin biosynthetic genes and evidence of multiple functional domains. J Bacteriol 173:2354–2365Google Scholar
  25. Henry MF, Cronan JE Jr (1992) A new mechanism of transcriptional regulation: release of an activator triggered by small molecule binding. Cell 70:671–679Google Scholar
  26. Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244Google Scholar
  27. Hori K, Yamamoto Y, Minetoki T, Kurotsu T, Kanda M, Miura K, Okamura K, Furuyama J, Saito Y (1989) Molecular cloning and nucleotide sequence of the gramicidin S synthetase 1 gene. J Biochem 106:639–645Google Scholar
  28. Hori K, Yamamoto Y, Tokita K, Saito F, Kurotsu T, Kanda M, Okamura K, Furuyama J, Saito Y (1991) The nucleotide sequence for a proline-activating domain of gramicidin S synthetase 2 gene from Bacillus brevis. J Biochem 110:111–119Google Scholar
  29. Joyard J, Stumpf PK (1981) Synthesis of long-chain acyl-CoA in chloroplast envelope membranes. Plant Physiol 67:259–256Google Scholar
  30. Kohara Y, Kiyotaka A, Katsumi I (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508Google Scholar
  31. Krätzschmar J, Krause M, Marahiel MA (1989) Gramicidin S biosynthesis operon containing the structural genes grsA and grsB has an open reading frame encoding a protein homologous to fatty acid thioesterases. J Bacteriol 171:5422–5429Google Scholar
  32. Liu J, Duncan K, Walsh CT (1989) Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dehydro-2,3dihydroxybenzoate dehydrogenase. J Bacteriol 171:791–798Google Scholar
  33. Lozoya E, Hoffmann H, Douglas C, Schulz W, Scheel D, Hahlbrock K (1988) Primary structures and catalytic properties of isoenzymes encoded by two 4-coumarate:CoA ligase genes in parsley. Eur J Biochem 176:661–667Google Scholar
  34. MacCabe AP, van Liempt H, Palissa H, Unkles SE, Riach MBR, Pfeifer E, von Döhren H, Kinghorn JR (1991) Delta-(l-aminoadipyl)-l-cysteinyl-d-valine synthetase from Aspergillus nidulans. J Biol Chem 266:12646–12654Google Scholar
  35. Mallonee DH, White WB, Hylemon PB (1990) Cloning and sequencing of a bile acid-inducible operon from Eubacterium sp strain VPI 12708. J Bacteriol 172:7011–7019Google Scholar
  36. Maniatis T, Fritsch EF, Sambrock J (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  37. Masuda T, Tatsumi H, Nakano E (1989) Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly Luciola cruciata. Gene 77:265–270Google Scholar
  38. Médigue C, Viari A, Henaut A, Danchin A (1991) Escherichia coli molecular genetic map (1500 kbp) update II. Mol Microbiol 5:2629–2640Google Scholar
  39. Messing J, Gronenborn B, Müller-Hill B, Hofschneider PH (1977) Insertion of a HindII fragment of the Lac regulatory region in M13 replicative form in vitro. Proc Natl Acad Sci USA 74:3642–3646Google Scholar
  40. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  41. Morris ME, Jinks-Robertson S (1991) Nucleotide sequence of the LYS2 gene of Saccharomyces cerevisiae: homology to Bacillus brevis tyrocidine synthetase 1. Gene 98:141–145Google Scholar
  42. Overath P, Pauli G, Schairer HU (1969) Fatty acid degradation in Escherichia coli. An inducible acyl-CoA synthetase, the mapping of old mutants, and the isolation of regulatory mutants. Eur J Biochem 7:559–574Google Scholar
  43. Rusnak F, Sakaitani M, Drueckhammer D, Reichert J, Walsh CT (1991) Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene expression and purification of EntF, and analysis of the covalent phosphopantetheine. Biochemistry 30:2916–2927Google Scholar
  44. Schmitz A, Gartemann K-H, Fiedler J, Grund E, Eichenlaub R (1992) Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU. Appl Environ Microbiol 58:4068–4071Google Scholar
  45. Scholten JD, Chang K-H, Babbitt PC, Charest H, Sylvestre M, Dunaway-Marino D (1991) Novel enzymatic hydrolytic dehalogenation of a chlorinated aromatic. Science 253:182–185Google Scholar
  46. Short JM, Fernandez JM, Sorge JA, Huse WD (1988) LambdaZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res 16:7583–7600Google Scholar
  47. Smith DJ, Earl AJ, Turner G (1990) The multifunctional peptide synthetase performing the first step of penicillin biosynthesis in Penicillium chrysogenum is a 421073 dalton protein similar to Bacillus brevis peptide antibiotica synthetases. EMBO J 9:2743–2750Google Scholar
  48. Staab JF, Elkins MF, Earhart CF (1989) Nucleotide sequence of the Escherichia coli entE gene. FEMS Microbiol Lett 59:15–20Google Scholar
  49. Steele MJ, Lorenz D, Hatter K, Parks A, Sokatch JR (1992) Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase. J Biol Chem 267:13585–13592Google Scholar
  50. Suzuki H, Kawarabayasi Y, Kondo J, Abe T, Nishikawa K, Kimura S, Hasimoto T, Yamamoto T (1990) Structure and regulation of rat long-chain acyl-CoA synthetase. J Biol Chem 265:8681–8685Google Scholar
  51. Tatsumi H, Kajiyama N, Nakano E (1992) Molecular cloning and expression in Escherichia coli of a cDNA clone encoding luciferase of a firefly Luciola lateralis. Biochim Biophys Acta 1131:161–165Google Scholar
  52. Turgay K, Krause M, Marahiel MA (1992) Four homologous domains in the primary structure of GrsE are related to domains in a superfamily of adenylate-forming enzymes. Mol Microbiol 6:529–546Google Scholar
  53. van Beilen J, Eggink G, Enequist H, Bos R, Witholt B (1992) DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans. Mol Microbiol 6:3121–3136Google Scholar
  54. Weckermann R, Fürbaß R, Marahiel MA (1988) Complete nucleotide sequence of the tycA gene coding the tyrocidine synthetase I from Bacillus brevis. Nucleic Acids Res 16:11841–11841Google Scholar
  55. Wood KV, Lam YA, Seliger HH, McElroy WD (1989) Complementary DNA encoding click beetle luciferases can elicit bioluminescence of different colors. Science 244:700–702Google Scholar
  56. Wu G, Williams HD, Zamanian M, Gibson F, Poole RK (1992) isolation and characterization of Escherichia coli mutants affected in aerobic respiration: the cloning and nucleotide sequence of ubiG. Identification of an S-adenosyl-methionine-binding motif in protein, RNA and small molecule methyltransferases. J Gen Microbiol 138:2101–2112Google Scholar
  57. Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A (1992) Systematic sequencing of the Escherichia coli genome: analysis of the 0–2.4 min region. Nucleic Acids Res 20:3305–3308Google Scholar
  58. Zhang J, Deutscher MP (1988) Escherichia coli RNase D: sequencing of the rnd structural gene and purification of the overexpressed protein. Nucleic Acids Res 16:6265–6278Google Scholar
  59. Zhao Y, Kung SD, Dube SK (1990) Nucleotide sequence of rice 4-coumarate: CoA ligase gene 4-CL1. Nucleic Acids Res 18:6144–6144Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Martin Fulda
    • 1
  • Ernst Heinz
    • 1
  • Frank P. Wolter
    • 1
  1. 1.Institut für Allgemeine BotanikUniversität HamburgHamburgGermany

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