Molecular and General Genetics MGG

, Volume 242, Issue 3, pp 241–249 | Cite as

The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal -map and is a new member of the AMP-binding protein family

  • Martin Fulda
  • Ernst Heinz
  • Frank P. Wolter
Original Articles


The fadD gene of Escherichia coli K12 was cloned and sequenced. The gene was identified by its ability to complement the corresponding mutant and by measuring the enzymatic activity after its expression in this mutant. The deduced polypeptide sequence exhibits similarity to other long chain acyl-CoA (coenzyme A) synthetases and a variety of other proteins, which together form a family of AMP-binding proteins. This family is extended by several new members and subdivided into four groups. fadD is assigned to a subgroup that does not include long chain acyl-CoA synthetases from eukaryotic organisms.

Key words

FadD acyl-CoA synthetase AMP-binding Escherichia coli K12 Sequence 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Martin Fulda
    • 1
  • Ernst Heinz
    • 1
  • Frank P. Wolter
    • 1
  1. 1.Institut für Allgemeine BotanikUniversität HamburgHamburgGermany

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