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Molecular and General Genetics MGG

, Volume 221, Issue 1, pp 125–128 | Cite as

Mapping of complementation groups within a Rhizobium leguminosarum CFN42 chromosomal region required for lipopolysaccharide synthesis

  • Joseph R. Cava
  • Hong Tao
  • K. Dale Noel
Article

Summary

A major genetic region specifying portions of the carbohydrate structure of Rhizobium leguminosarum CFN42 lipopolysaccharide was analyzed by Tn5 mutagenesis, constructing deletions in cloned DNA, restriction mapping, and complementation analysis. Mutations affecting lipopolysaccharide synthesis were arranged in nine complementation groups spanning 18 kb of DNA. One mutation resulted in O-polysaccharide-containing lipopolysaccharide having a slightly increased mobility in gel electrophoresis. This mutation did not affect the symbiosis with bean plants. The other mutations eliminated the 0-polysaccharide-containing lipopolysaccharide and resulted in strains defective in eliciting bean nodule development.

Key words

Rhizobium Lipopolysaccharide Genetic complementation Nodule development 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Joseph R. Cava
    • 1
  • Hong Tao
    • 1
  • K. Dale Noel
    • 1
  1. 1.Department of BiologyMarquette UniversityMilwaukeeUSA

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