Molecular and General Genetics MGG

, Volume 221, Issue 1, pp 33–36

Coding sequence and flanking regions of the mouse vimentin gene

  • Hartwig Hennekes
  • Siegfried Kühn
  • Peter Traub
Article

DOI: 10.1007/BF00280364

Cite this article as:
Hennekes, H., Kühn, S. & Traub, P. Molec. Gen. Genet. (1990) 221: 33. doi:10.1007/BF00280364

Summary

Using a polyclonal antibody, a cDNA clone coding for part of mouse vimentin was identified in a λgt11 expression library. DNA from this clone was used to screen a genomic library from Ehrlich Ascites Tumor cells for the mouse vimentin gene. A clone was found which contained the whole coding sequence and a large part of the 5′- and 3′-untranslated sequences. It was used to prepare a construct equivalent to a full-length cDNA clone. Extensive homologies to the vimentin sequence from other species were found for the coding and 3′-untranslated sequences and the promoter region.

Key words

Intermediate filament protein Vimentin Promoter 

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Hartwig Hennekes
    • 1
  • Siegfried Kühn
    • 1
  • Peter Traub
    • 1
  1. 1.Max-Planck-Institut für ZellbiologieLadenburg bei HeidelbergGermany

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